溶血卵磷脂对血管内皮细胞增殖及凋亡的影响

目的观测溶血卵磷脂(LPC)对血管内皮细胞增殖及凋亡的影响,从而探讨动脉硬化发生的机制。方法取体外培养的人脐静脉内皮细胞(HUVECs),在含不同浓度LPC(5、10、20mg/L)的培养基中分别培养6、12、24、48h,用四甲基偶氮唑蓝(MTT)比色法、流式细胞仪、荧光显微镜观测血管内皮细胞增殖及凋亡的变化。结果与正常对照组比较,LPC抑制内皮细胞增殖,促进细胞凋亡,且其作用呈时间-效应、浓度-效应依赖关系,但随着LPC浓度增加,细胞凋亡增加的同时细胞死亡比例也增加。结论LPC抑制血管内皮细胞增殖,促进细胞凋亡,可能是其致动脉粥样硬化机制之一。

Effects on proliferation and apoptosis of cultured human umbilical vein endothelial cells induced by lysophosphatidylcholine

Objective To investigate the effects on proliferation and apoptosis of HUVECs induced by LPC.Methods HUVECs were treated with different concertrations of LPC(5,10,20 mg/L)for 6,12,24,48 h respectively.Proliferation and apoptosis of HUVECs were assessed by MTT assay,flow cytometry(FCM)and fluorescence microscopy respectively.Results Compared with control group,LPC could inhibit the proliferation and induce apoptosis of HUVECs in a dose-dependent and time-dependent way.Meanwhile,the death of the cells was increased with the increasing of LPC concentration.Conclusion LPC could inhibit the proliferation and induce apoptosis of HUVECs,which may be one of mechanisms responsible for LPC to lead to atherosclerosis.