竹叶黄酮对大鼠肝脏缺血再灌注损伤的影响及机制研究

目的：观察竹叶黄酮对大鼠肝脏缺血再灌注损伤的保护作用并探讨其作用机制。方法将SD大鼠按随机数字表法分为假手术组、模型组、竹叶黄酮组。竹叶黄酮组大鼠腹腔注射竹叶黄酮溶液30 mg/kg，假手术组及模型组大鼠腹腔注射等体积生理盐水，1次/d，连续注射14 d。末次给药次日，模型组及竹叶黄酮组建立大鼠肝脏缺血再灌注模型。于再灌注后6 h，检测各组大鼠血清ALT、AST水平；采用ELISA法检测各组大鼠血清IL-1、IL-6水平；采用HE染色观察各组大鼠肝组织病理学改变；采用Western Blot检测肝组织半胱氨酸天冬氨酸蛋白酶-3(caspase-3)表达。结果与模型组比较，竹叶黄酮组大鼠血清 ALT(343.11±78.32)U/L 比(215.31±52.21)U/L]、AST(863.97±63.58)U/L 比(501.59±70.13)U/L]、IL-1(596.85±102.34)U/L 比(315.30±88.64)U/L]、IL-6(183.64±20.69)U/L 比(106.75±10.25)U/L]水平降低(P＜0.05)，竹叶黄酮组大鼠肝组织caspase-3(0.38±0.05)比(0.86±0.09)]表达水平降低(P＜0.05)。结论竹叶黄酮预处理可减轻大鼠肝脏缺血再灌注损伤，其作用机制可能与抑制caspase-3蛋白表达有关。

The influence and mechanism research of bamboo leaf flavone on hepatic ischemia- reperfusion injury in rats

Objective To explore the protective effect of bamboo leaf flavone in hepatic ischemia-reperfusion injury (HIRI).Methods SD rats were divided into the sham group, the HIRI group and the bamboo leaf flavone intervention group. Rats in bamboo leaf flavone intervention group received bamboo leaf flavone (30 mg/kg) by intraperitoneal injection for 14 consecutive days before establishing the model of hepatic ischemia-reperfusion. Rats in the sham group and the HIRI group received the same volume normal saline. The serum alanine aminotransferase (ALT) and aspertate aminotransferase (AST) were detected. The serum IL-1, IL-6 were detected by ELISA. The expression of caspase-3 in liver tissue was detected by the Western Blot.Results Compared to the HIRI group, the ALT (215.31 ± 52.21 U/Lvs.343.11 ± 78.32 U/L), AST (501.59 ± 70.13 U/Lvs.863.97 ± 63.58 U/L), IL-1 (315.30 ± 88.64 U/Lvs.596.85 ± 102.34 U/L), IL-6 (106.75 ± 10.25 U/L vs. 183.64 ± 20.69 U/L) in the bamboo leaf flavone intervention group significantly decreased (P<0.05). Compared to the HIRI group, expression of caspase-3 (0.38 ± 0.05vs. 0.86 ± 0.09) in the bamboo leaf flavone intervention group significantly decreased (P<0.05).Conclusions Bamboo leaf flavone pretreatment could reduce HIRI and the mechanism may be associated with inhibiting the expression of caspase-3 protein.