结直肠癌组织中B细胞集落增强因子基因表达水平及其临床意义

目的：探讨前 B 细胞集落增强因子（PBEF）基因在结直肠癌中的表达情况，分析其与结直肠癌临床病理特征的关系以及诊断和预后价值。方法采用免疫组织化学 SP 法，检测68例结直肠癌组织标本及其癌旁正常组织中 PBEF 的表达水平，分析 PBEF 表达水平与肿瘤病理特征及预后之间的相关性。结果结直肠癌组织和正常肠黏膜组织中 PBEF 的表达阳性率分别为80．9％（55／68）和29．4％（20／68），两组间差异有统计学意义（χ2＝36．41，P ＜0．05）。结直肠癌组织中 PBEF 表达水平与患者年龄（χ2＝0．11，P ＞0．05）、性别（χ2＝0．39，P ＞0．05）、组织学类型（χ2＝0．54，P ＞0．05）、是否穿透浆膜（χ2＝1．55，P ＞0．05）无关，但与分化程度（χ2＝6．29，P ＜0．05）、淋巴结转移（χ2＝4．32，P ＜0．05）、远处转移（χ2＝4．16，P ＜0．05）及临床分期（χ2＝7．58，P ＜0．05）有关。PBEF 蛋白表达阳性、阴性患者的5年生存率分别为73．3％、81．8％，两组差异无统计学意义（χ2＝0．315，P ＞0．05）。结论PBEF 异常高表达可能与结直肠癌的发生、发展密切相关。

Pre-B-cell colony enhancing factor expression and its clinical significance in colorectal cancer

Objective To explore the expression of pre-B-cell colony enhancing factor (PBEF)in colorectal cancer,and analyze the relationship between PBEF expression and the clinical pathological characte-ristics and its diagnostic and prognostic value.Methods Sixty-eight human colorectal tumor and normal colo-rectal tissues were collected,and the expression of PBEF was detected by immunohistochemical method.The relationship between the expression level of PBEF and the characteristics of tumors and prognosis was analyzed. Results The positive expression rate of PBEF in colorectal cancer tissues was 80.9%,higher than that in nor-mal colorectal tissues,which was 29.4% (χ2 =36.41 ,P <0.05).The expression level of PBEF had no rela-tionship with age (χ2 =0.1 1 ,P >0.05),sex (χ2 =0.39,P >0.05),histology type (χ2 =0.54,P >0.05) or penetrating serosa (χ2 =1 .55,P >0.05),but significantly associated with the degree of differentiation (χ2 =6.29,P <0.05),metastasis of lymph node (χ2 =4.32,P <0.05),distant metastasis (χ2 =4.1 6,P <0.05)and clinical stage (χ2 =7.58,P <0.05).The 5 year survival rates of patients with positive and nega-tive expression of PBEF were 73.3% and 81 .8%,and the difference was not statistically significant (χ2 =0.31 5,P >0.05).Conclusion The abnormal high expression of PBEF may be closely related to the occur-rence and development of colorectal cancer.