疏肝法对围绝经期综合征肝郁证模型大鼠雌激素受体mRNA表达的影响

目的：观察疏肝法对围绝经期综合征(perimenopausal syndrome, PMS)肝郁证模型大鼠行为学及其卵巢雌激素受体(Estrogen receptor, ER)mRNA表达的影响，探讨其作用机制。方法将围绝经期大鼠按随机数字表法分为模型组、柴胡疏肝散组、丹栀逍遥散组，每组8只。采用孤养法联合慢性束缚法制备PMS肝郁证大鼠模型。另将8只3月龄SD健康大鼠作为正常对照组。柴胡疏肝散组大鼠灌胃柴胡疏肝散水煎剂4.0 g/kg，丹栀逍遥散组灌胃丹栀逍遥散水煎剂4.9 g/kg，模型组及正常对照组灌胃等体积生理盐水，1次/d，连续给药21 d。采用旷场实验观察各组大鼠行为学变化；采用放射性免疫技术检测大鼠血清雌二醇、卵泡刺激素及促黄体生成素水平；采用实时荧光定量PCR法检测大鼠卵巢组织ERα、ERβ mRNA表达变化。结果给药后21 d，与模型组比较，柴胡疏肝散组、丹栀逍遥散组大鼠水平运动得分[(49.6±6.0)分、(51.6±5.8)分比(40.0±4.6)分]、垂直运动得分[(14.1±0.7)分、(14.6±2.3)分比(10.9±1.8)分]提高(P＜0.05及P＜0.01)；柴胡疏肝散组大鼠卵巢ERα mRNA[(7.42±2.54)比(3.80±1.36)]表达水平升高(P＜0.01)，血清卵泡刺激素[(3.96±0.48)mIU/ml比(5.31±0.41)mIU/ml]水平降低(P＜0.01)；丹栀逍遥散组大鼠血清促黄体生成素[(6.65±0.46)mIU/ml 比(8.10±0.62)mIU/ml]水平降低(P＜0.01)。结论疏肝法可改善 PMS 肝郁证大鼠的行为学，其治疗机制可能与调节内分泌及提高卵巢雌激素受体基因表达相关。

Effects of liver-soothing therapy on the expression of estrogen receptors mRNA in perimenopausal syndrome rats with liver qi stagnation

Objective To explore the effect of liver-soothing therapy on the expression of estrogen receptors mRNA in perimenopausal syndrome (PMS) rats with liver qi stagnation. Methods A total of 30 nature aging rats are assigned into control groups (n=8), model groups (n=8),Chaihu-Shugan San (CHSGS group,n=8) andDanzhi-Xiaoyao San (DZXYS group, n=8), according to the random number table. The PMS liver-Qi stagnation syndrome rat models were established by the methods of isolation raised and chronic bondage in all the groups except the control group. CHSGS group were administered 4.0 g/kg water decoctions ofChaihu ShuganSan, and DZXYS group 4.9 g/kg water decoctions ofDanzhi XiaoyaoSan respectively for 3 weeks after the rat models established. The model group and control group were administered with equal volume of normal saline. The open field test was used for the behavior test. The serum E2, FSH, LH level were measured by radioimmunoassay. The ERα, ERβ in ovary were measured by quantitative real-time PCR. Results Compared with model group on the 21st days, the CHSGS and DZXYS groups showed a significantly increase in crossings (49.6 ± 6.0, 51.6 ± 5.8vs. 40.0 ± 4.6,P<0.05 orP<0.01) and rearings (14.1 ± 0.7, 14.6 ± 2.3vs. 10.9 ± 1.8,P<0.05 orP<0.01). Cmpared with the model group, the FSH (3.96 ± 0.48 mIU/mlvs.5.31 ± 0.41 mIU/ml) significantly decreased in the CHSGS group, and the LH (6.65 ± 0.46 mIU/mlvs. 8.10 ± 0.62 mIU/ml) significantly decreased in the DZXYS group (P<0.05). Compared with the model group, ER alpha mRNA expression (7.42 ± 2.54, 4.91 ± 1.76vs. 3.80 ± 1.36,P<0.01) significantly increased in the CHSGS group, and the ER beta mRNA expression (3.56 ± 0.95vs. 3.10 ± 1.12,P>0.05) increaed in the DZXYS group, but there was no remarkable difference. Conclusion The Liver-soothing therapy can improve the behavior of PMS rats with liver-Qi stagnation, and the mechanism may be related to the regulation of endocrine and ovarian estrogen receptors.