Replication of total and mitochondrial DNA in rat liver with aging |
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| Authors: | Alexander Ya. Litoshenko Eugene L. Levitsky |
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| Affiliation: | 1. Laboratory of Biochemistry, Institute of Gerontology AMS U.S.S.R., Kiev, U.S.S.R.;1. Laboratory of Molecular Biology, Institute of Gerontology AMS U.S.S.R., Kiev, U.S.S.R. |
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| Abstract: | Replication of total (t-DNA) and mitochondrial (mt-DNA) liver DNA of adult (6 months) and old (24 months) female rats labelled with i.v. 3H-thymidine has beeb studied. The most part of t-DNA radioactivity was shown to result from the replication of nuclear DNA (n-DNA). 10, 30, 70 and 120 min after labelling, intracellular acid-soluble 3H-radioactivity of old rats was significantly higher than that of adult ones. This may be due to the change in precursors concentration with aging. Thus, despite the lack of differences between the specific radioactivities of t-DNA and mt-DNA of adult and old animals during all labelling periods used the true replication rate, i.e., specific DNA radioactivity/volume of intracellular pool of label (relative DNA radioactivity), was significantly higher in adult animals during all labelling periods.Decrease of n-DNA and mt-DNA replication rate may lead to lowering of energetic potential and cell renewal in the liver with aging. |
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