rhIL-11不同时间给药对急性放射病猕猴造血系统的影响

目的 观察rhIL-11不同时间约药对^60Coγ射线照射猕猴造血系统的影响。方法 正常成年猕猴用^60Coγ射线一次全身照射3．0Gy ，吸引剂量率154．96cGy/min，分照射对照、早期给药和后给药3组，后两组分别在照射后当天和第13天开始皮下注射rhIL-1160μg.kg^-1.d^-1(μKD)，每日1次，连续14d。结果 照射后应用rhIL-11可明显短照射猴外周血小板数和白细胞数量低值的持续时间，早期给药还可明显升高照射猴外周血小板数的最低值。给药后1周内，早期给药组外周血红细胞数较照射对照组有一定程度的降低，从给药后第19天开始则明显高于照射对照组。照射后45d骨髓细胞培养结果表明，rhIL-11可明显刺激照射猴骨髓有核细胞体外形成CFU－MK和CFU－Mix的能力。病理组织学观察结果同样表明 IL-11治疗组骨髓粒系和巨核系细胞明显增殖。结论 rhIL-11可明显促进急性放射病猴骨髓造血功能的恢复，照射后早期给药的效果优于第13天开始给药。

Therapeutic effect of rhIL-11 administered at different times on acute radiation sickness in rhesus monkeys

Objective　This report describes the therapeutic effect of recombinant human interlukine-11(rhIL-11)administered at different times on acute radiation sickness in monkeys. MethodsRhesus monkeys irradiated with 3.0 Gy 60Co γ-rays were divided into 3 groups.One group was the control administered with vehicle,the second one was subjected to administer rhIL-11 on days 0-13 after TBI(60 μg*kg-1*d-1,sc) and the third one to administer rhIL-11 on days 13-26 after TBI at the same doses. Results　 The early treated group had higher platelet nadirs compared with that of the other two.The duration of platelet and leukocyte numbers below 50% of their baseline values shortened significantly in animals treated with rhIL-11,especially in the early treated group.During the first week after irradiation,the early treated group had lower erythrocyte count compared with the control,but it began to rise at day 19 after irradiation.Semi-solid bone marrow cell culture demonstrated that rhIL-11 could stimulate bone marrow cells to form more CFU-MK,CFU-Mix,CFU-E,BFU-E and CFU-GM in vitro.We also got the same results in histopathological observation. Conclusion　rhIL-11 administered at different times can not only accelerate the haemotopoietic recovery of acute radiation sickness in rhesus monkeys,but also result in better therapeutic effect when administered earlier.