不同种类离体皮肤及人工合成膜对川芎贴膏体外透皮试验的影响

目的 对川芎贴膏体外透皮试验用皮肤作比较研究，选择适宜亲水性成分的实验皮肤并对离体皮肤的前处理方法加以规范。初步为中药经皮给药制剂体外扩散试验评价体系的建立奠定基础。方法 应用TP-5型智能透皮扩散仪，以川芎贴膏中盐酸川芎嗪为考察指标，采用紫外分光光度法测定，比较不同种类离体皮肤及人工合成膜对川芎贴膏经皮渗透参数的影响。结果 累积渗透量及透皮速率：硝酸纤维素膜〉裸鼠〉小鼠〉大鼠〉兔〉硅橡胶〉乳猪〉蛇蜕；时滞：硅橡胶〉硝酸纤维素〉蛇蜕〉小鼠〉兔〉裸鼠〉大鼠〉乳猪。结论 硝酸纤维素膜可作为适宜亲水性成分盐酸川芎嗪体外经皮渗透实验的理想皮肤模型。

Comparation of permeability of Chuan Xiong adhesive plaster on excised skins and synthetic membranes in vitro

ABSTRACT: AIM To assess the percutaneous permeability of ChuanXiong Plaster in the comparison of different animal skins and synthetic membranes. METHODS TP-5 automatic diffusion apparatus was adopted for in vitro skin permeation. The permeat experiments were accomplished by testing the deposit of ligustrazine hydrochloride onto various animal skins and synthetic membranes. RESULTS The order of cumulative amount and permeat rate: pyroxylin hairless mouse>mouse>rat>rabbit>slicone rubber>porket>smake slough. Lag time: silicone rubber>pyroxylin>snake slough>mouse>rabbit>hairless mouse>rat>porket. CONCLUSION Cellulose nitrate filtercan be regarded as good model for the study of percutanous of Ligustrazine hydrochloride.