SIVmac239对恒河猴B淋巴细胞生长繁殖的抑制作用

目的探讨恒河猴免疫缺陷病毒SIVmac239感染CD4 T淋巴细胞后可否产生某些抑制B淋巴细胞的生长活性因子,为HIV/AIDS进一步的病原学研究和控制提供有益的线索。方法用MTT实验观察不同时间收集的SIVmac239感染的CD4 T淋巴细胞(C8166)上清和未感染SIVmac239的CD4 T淋巴细胞的上清对恒河猴B淋巴细胞(MM133)增殖的抑制作用情况。结果SIVmac239感染的CD4 T淋巴细胞(C8166)上清含有抑制猕猴B淋巴细胞生长的因子,且其抑制作用效果随着作用时间的延长而增加。结论SIVmac239感染CD4 T淋巴细胞(C8166)可产生抑制B淋巴细胞(MM133)生长的因子。

Inhibition of SIVmac239 on macques B lymphocyte (MM133) proliferation in vitro

Objective To explore the inhibition factors which CD4~( )T lymphocyte secret after its infection with SIVmac239,whether or not these factors could inhibit the B lymphocyte(MM133) proliferation activity,and to supply some helpful clue for pathogenology research of AIDS.Mehtods Proliferation activity of macques B lymphocyte(MM133) treated by the supernatant of C8166 cells infected SIVmac239 at different time was tested by MTT assay.The supernantant of C8166 cells without infection by SIVmac239 was inhibited.Results SIVmac239 could stimulate CD4~( )T lymphocyte(C8166) to secret some inhibitant factors for the macques B lymphocyte(MM133),and the inhibition effect increased by the culture time prolong.Conclusions CD4~( )T lymphocyte(C8166) infected by SIVmac239 could produce some inhibitant factors on the macques B lymphocyte(MM133).