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| 正交试验法优选连翘酯苷硅胶柱层析提取工艺 | |
| 引用本文： | 王科蕊,杨骏,黄孝春,玄振玉. 正交试验法优选连翘酯苷硅胶柱层析提取工艺[J]. 药学服务与研究, 2011, 11(5): 370-372. DOI: 10.5428/pcar20110516 |
| 作者姓名： | 王科蕊 杨骏 黄孝春 玄振玉 |
| 作者单位： | 1. 上海交通大学医学院附属第三人民医院药剂科,上海,201900 2. 上海卢湾区香山中医医院药剂科,上海,200020 3. 上海玉森新药开发有限公司研发部,上海,201203 |
| 基金项目： | 重大新药创制国家科技重大专项资助项目(No.2009ZX09313-008); 上海市优秀学科带头人计划资助项目(No.10XD1423100) |
| 摘 要： | 目的:优化用硅胶柱层析提取连翘叶中连翘酯苷的最佳提取工艺。方法:采用L9(34)正交试验设计,考察上样量(A)、洗脱液流速(B)和柱床径高比(C)对连翘酯苷得率的影响,优选最佳提取工艺条件。结果:上样量、洗脱液流速和柱床径高比对连翘酯苷得率的影响大小依次为:C>A>B,最佳工艺为样品与硅胶用量比1∶20,洗脱液流速1.5BV/h,柱床径高比1∶10。结论:该制备工艺简便、稳定和可行。 |
| 关 键 词： | 连翘酯苷 正交试验 提取工艺 硅胶柱层析 |
Optimization of the extraction process for silica gel column chromatography of forsythiaside by orthogonal test |
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| WANG KeRui,YANG Jun,HUANG XiaoChun,XUAN ZhenYu. Optimization of the extraction process for silica gel column chromatography of forsythiaside by orthogonal test[J]. Pharmaceutical Care and Research, 2011, 11(5): 370-372. DOI: 10.5428/pcar20110516 | |
| Authors: | WANG KeRui YANG Jun HUANG XiaoChun XUAN ZhenYu |
| Affiliation: | WANG KeRui1,YANG Jun2,HUANG XiaoChun3,XUAN ZhenYu3(1.Department of Pharmacy,The Third People's Hospital affiliated to School of Medicine,Shanghai Jiaotong University,Shanghai 201900,China,2.Department of Pharmacy,Xiangshan Chinese Medicine Hospital of Shanghai Luwan District,Shanghai 200020,3.Department of Research and Development,Shanghai Youseen Drug Development Corporation,Shanghai 201203,China) |
| Abstract: | Objective:To optimize the extraction process of forsythiaside by silica gel column chromatography.Methods:Orthogonal design [L9(34)] was used to select the best extraction process.The loading quantity of sample(A),eluent flow rate(B) and diameter-height ratio of column(C) were set as indexes for orthogonal test.Results: Diameter-height ratio of column(C) was the first influential factor,the loading quantity(A) of sample was the second and the eluent flow rate(B) was the third.The optimal extraction process ... |
| Keywords: | forsythiaside orthogonal test extraction process silica gel column chromatography |
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