马来酸罗格列酮干预高脂饲养大鼠脂肪肝的研究

目的观察高脂饲养所致正常SD大鼠脂肪肝与胰岛素抵抗的关系及罗格列酮干预脂肪肝的效果。方法将8周龄雄性SD大鼠随机分为三组，每组各11只：正常饲养组（NC）、高脂饲养组（HF）、高脂＋罗格列酮组（HF4-Ros）。饲养8周后取空腹血测定血糖、脂联素，胰岛素敏感性用正常血糖高胰岛素钳夹术稳态时的葡萄糖输注率（GIR）来评价，测定肝脏中TG含量，并对大鼠肝脏组织做HE染色。结果高脂饲养8周后，与NC组相比，HF组肝脏呈现明显脂肪肝，肝脏中TG明显增加达154．2％（NC组3．89mg／g，HF组9．89mg／g，P〈0．01）；与HF组相比，HF4-Ros组肝脏中TG显著降低达46．6％（5．28mg／g，P〈0．01），脂肪肝减轻。结论马来酸罗格列酮能明显改善高脂饲养大鼠的脂肪肝。

Effects of rosiglitazone on fatty liver induced by high fat diet in rats

Objective To observe the relationship between fatty liver and insulin resistance(IR) and effects of rosiglitazone on fatty liver induced by high-fat diet (HFD) in rats. Methods Normal male SD rats aged 8-weeks were divided into three groups:normal chow group (NC, n=11), high-fat diet (HF, n=11)and rosiglitazone-treated group (HF Ros, n=11). After feeding for 8 weeks, serum fasting glucose, adiponectin and TG content of liver(LTG) were measured in all rats. Insulin sensitivity was measured with glucose infusion rate (GIR) during euglycemic-hyperinsulinemic clamp. Results HFD induced fatty liver in SD rats after feeding for 8 weeks. LTG increased significantly by 154.2%(3.89 mg/g vs 9.89 mg/g in NC vs HF group respectively,P<0.01). When compared with HF group, LTG was decreased by 46.6% (5.28 mg/g vs 9.89 mg/g,P<0.01) in HF Ros group. Conclusions Rosiglitazone could improve liver steatosis induced by fat-rich diet.