鸡Ⅱ型胶原蛋白的药物动力学研究

目的研究了鸡Ⅱ型胶原（chicken typeⅡcollagen, CCⅡ）在大鼠体内的药物动力学。方法采用氯胺T法进行125I-CCⅡ标记，放化纯度>96.26%。给大鼠灌胃7.4×105、1.48×106 及2.96×106 Bq*kg－1 的125I-CCⅡ，然后测定9个时间点的血药浓度，用一室模型进行参数计算。大鼠灌胃2.96×106 Bq*kg－1后，测定1 、3 、6 h的分布及排泄情况。结果125I-CCⅡ的吸收相较短，t1/2Ka约为0.56～0.74 h。消除较快，t1/2Ke约为3.60～4.32 h。该药分布较快，以肾、胃、肠、肺、肝、脾的分布量较高。在72 h 内从尿及粪中分别排出27.9%和4.8%，26 h 内从胆汁中排出6.7%。结论CCⅡ口服给药后，吸收较快，消除也较迅速，并且分布广泛。

PHARMACOKINETICS OF CHICKEN TYPE Ⅱ COLLAGEN IN RATS

OBJECTIVE:To study the pharmacokinetic characteristics of chicken type Ⅱ collagen (CC-Ⅱ) in rats.METHODS:To use Chloramines-T method to label CCⅡ by 125　I, the radiochemical purity is more than 96.26%. Administrated orally with three different doses (7.4×105、1.48×106、2.96×106 Bq*kg-1) of 125　I-CCⅡ, 9 time points of blood concentrations were detected. The pharmacokinetic parameters were calculated by 3p87 program and the radioactive drug plasma concentration fitted well to one compartment model. The blood, urine, bile, feces and tissue sample are collected and assessed for excretion and distribution.RESULTS:The results show that the absorbing time of CC Ⅱ is short (t1/2Ka is 0.56～0.74 h), the elimination are quick (t1/2Ke is 3.60～4.32 h). Tissue distribution in kidney, stomach, intestine, lung, liver and spleen are higher than others after 1 h. Compared to the total doses administrated, 27.9% of the radian excreted in urine and 4.8% in feces in 72 h and 6.7% in bile with in 26 h.CONCLUSION:After oral administration, CC Ⅱ is rapidly absorbed and it distributes broadly in the tissue.