Isoenzymes of γ-glutamyl transpeptidase in liver diseases |
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Authors: | E Bruch Igartua R Domecq J Findor |
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Institution: | (1) Hospital de Clinicas José de San Martin, Division of Clinical Enzymology, University of Buenos Aires, Argentina |
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Abstract: | Summary A new method for the separation of isoenzymes of-glutamyl-transpeptidase is described, using electrophoresis on acetate cellulose gel and a developing solution composed by-glutamyl-naphthylamide, and a colored diazonium compound.The method permits the separation of up to four different isoenzymes, which we called-GT1,-GT2,-GT3,-GT4, the first two showing an electrophoretic migration similar to that of
1- and
2-globulins and the other two to that of-globulins.The present technique has proved its usefulness in detecting isoenzymes in serum with values of total-glutamyl-transpeptidase higher than 80 U/L.The application of this method in 52 patients with different types of biliary obstruction and hepatocellular damage has shown that it provides new possibilities in differential diagnosis. |
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Keywords: | Enzymology -glutamyl-transpeptidase" target="_blank">gif" alt="gamma" align="MIDDLE" BORDER="0">-glutamyl-transpeptidase isoenzymes differential diagnosis of jaundice |
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