人巨细胞病毒感染对脐血巨核系祖细胞体外增殖的影响

目的　探讨人巨细胞病毒 (HCMV)对脐血巨核系祖细胞 (CFU Mk)体外增殖的影响及其机制。方法　采用造血祖细胞体外培养技术 ,观察、计数HCMV AD169感染的CFU Mk集落产率、抑制率、集落峰值及维持时间 ;并采用聚合酶链反应 (PCR)技术检测集落细胞内HCMV AD169DNA。结果　 1.HCMV AD169感染组集落产率较对照组明显减少 ,HCMV对CFU Mk集落的抑制程度与病毒滴度有关 ,集落产率随病毒感染滴度增高而减少 ,抑制率随病毒感染滴度增高而渐增加 ;各组集落峰值出现时间无明显差异 (P均 >0 .0 5 ) ,但各感染组集落维持时间明显短于对照组 (P <0 .0 1) ;2 .用PCR在集落细胞内检测到HCMV AD169DNA。结论　CFU Mk是HCMV的宿主细胞之一 ,HCMV能直接感染CFU Mk ;在体外HCMV AD169对CFU Mk的增殖和集落形成有明显抑制作用 ,此作用可能与临床HCMV感染后患者血小板减少有关

The effect of human cytomeglavirus on proliferation of megakaryocyte progenitor cells in vitro

Objective To investigate the mechanism and suppression effect of human cytomegalovirus (HCMV) on proliferation of megakaryocyte progenitors(CFU-Mk)in vitro.Methods Colony forming unit-assay was applied to observe the effect of HCMV-AD 169 strain on CFU-Mk of cord blood. The technique of PCR was used to demonstrate the existence of HCMV-AD 169 DNA in the colony cells of cultured CFU-Mk.Results 1.The number of CFU-Mk colonies in HCMV-infected groups decreased significantly compared with that of control group. The CFU-Mk formation was inhibited significantly after HCMV-AD 169 strain infection.The suppression effect showed a dose-dependent fashion: 46.7 % inhibition with 10 -1of HCMV, 29.7 % with 10 -2 and 14.5 % with 10 -3 in the CFU-Mix assay. The peak of CFU-Mk colonies (d16-18) was not significantly different between control group and experimental groups, but the duration of the CFU-Mk colonies in infected groups was significantly shorter than that in control group.2. HCMV-DNA was positively detected in the colony cells of viral infected group by PCR, while negative in control group.Conclusions HCMV-AD 169 strain may inhibit the differentiation and proliferation of CFU-Mk by infecting the hematopoietic progenitors. HCMV may cause the suppression of hematopoiesis by direct infection, which may be the main reason for HCMV infection associated with thrombocytopenia.