原发性胃癌组织中的端粒及端粒酶表达

目的观察端粒（ＴＲＦ）及端粒酶在胃癌形成及发展中的作用。方法采用ＤＮＡ琼脂糖凝胶杂交技术及端粒重复扩增ＰＣＲ方法检测１７例早期胃癌、８９例进展期胃癌组织及邻近正常组织中的平均ＴＲＦ长度及端粒酶活性。结果早期、进展期胃癌的ＴＲＦ长度及端粒酶活性表达均显著短于或高于邻近胃组织（Ｐ＜０．０５～０．０１），且端粒酶活性的表达主要表现在ＴＲＦ缩短或延长的胃癌组织中，而ＴＲＦ缩短与胃癌的分化程度相一致。结论端粒及端粒酶的异常状态可能与胃癌的发生发展密切相关，端粒酶活性及端粒缩短可以作为胃癌的诊断标志

Expression of telomere and telomerase in human primary gastric carcinoma

Objective To investigate the expression of telomere restriction fragments (TRF) and telomerase activity in human primary gastric carcinoma tissues and their role in tumor transformation and progression. Methods The lengths of TRF and activity of telomerase were observed in 17 early gastric carcinoma tissues and 89 advanced gastric carcinoma tissues, using hybridization of nucleic acids directly in agarose gels and telomere repeat amplification protocol (TRAP) assays, corresponding normal gastric mucosas were used as controls. Results The TRF lengths and telomerase activity in gastric cancer tissues were significantly shorter or higher than those in non tumor mucosa, and the expression of telomerase activity in abnormal TRF tumor tissues was significantly higher than that in normal TRF tumour tissues. Alterations of TRF and telomerase activity in advanced tumour tissues were higher than those in early tumor tissues. Moreover, significant differences in TRF length were observed between well differenciated and poorly differentiated adenocarcinomas. Conclusion Abnormal TRF status and telomerase reactivation may correlate well with malignant progression of gastric cancer. Telomerase activity and TRF lengths may thus serve as an important additional marker for tumor diagnosis.