| Abstract: | OBJECTIVE: Lansoprazole (Takepron; Takeda) is extensively used in the treatment of acid‐related diseases. There are no prior studies of the effects of antisecretion drugs on parietal cells, but we recently developed a rabbit parietal cell model. This allowed us to compare the antisecretory effects of lansoprazole and cimetidine in vitro in the present study. METHODS: Parietal cells were isolated using cell elutriation and continuous density gradient centrifugation. The effect of cimetidine and lansoprazole on histamine‐induced acid secretion in rabbit parietal cells was investigated by measuring the accumulation of 14C‐aminopurine. RESULTS: The purity and viability of isolated parietal cells was >80 and 95%, respectively. Lansoprazole and cimetidine both significantly inhibited histamine‐stimulated acid secretion (10?4 mol/L), but the 50% inhibition concentration (IC50) of the two treatments was quite different. The IC50 of cimetidine was 3.70 × 10?5 mol/L, and that of lansoprazole was 9.59 × 10?8 mol/L. CONCLUSIONS: Lansoprazole is much more effective than cimetidine in the in vitro inhibition of histamine‐stimulated acid secretion in rabbit parietal cells. This study developed a model for the study of acid inhibition drugs. |
