| 不同核酸提取方法和新型冠状病毒核酸检测试剂室间质评结果分析 |
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| 引用本文： | 余汉忠,牛璐璐,孔倩倩,李小川,蔡佩君,王丽.不同核酸提取方法和新型冠状病毒核酸检测试剂室间质评结果分析[J].临床检验杂志,2021,39(2):146-147. |
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| 作者姓名： | 余汉忠 牛璐璐 孔倩倩 李小川 蔡佩君 王丽 |
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| 作者单位： | 中国矿业大学附属医院徐州市第一人民医院检验科,江苏徐州221009;中国矿业大学化工学院,江苏徐州221009 |
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| 基金项目： | 2020年中国矿业大学新型冠状病毒(2019-nCoV)防治科研攻关应急专项资助项目(2020XGYJ02)。 |
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| 摘 要： | 目的 分析2种不同核酸提取方法和2种新型冠状病毒核酸检测试剂检测室间质评样本的结果,进行性能比较.方法收集2020年江苏省临床检验中心和国家卫生健康委临床检验中心发放的新型冠状病毒核酸检测室间质量评价样本共15份,用2种常用核酸提取方法(磁珠法、核酸释放剂法)、2个厂家实时荧光定量PCR核酸扩增检测试剂检测,进行质评物...
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| 关 键 词： | 新型冠状病毒 室间质评 核酸检测 核酸提取方法 |
| 收稿时间： | 2020/9/14 0:00:00 |
| 修稿时间： | 2021/3/22 0:00:00 |
Analysis of 2 novel coronavirus nucleic acid test methods and 2 new nucleic acid detection reagents for the detection of external quality assessment |
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| Abstract: | Objective 2 novel coronavirus nucleic acid extraction methods and 2 new coronavirus nucleic acid reagents were used to test the quality of the external quality assessment, and the performance comparison and analysis were carried out for other laboratories to make reference.
Method The first novel coronavirus nucleic acid test in Jiangsu province in 2020 and the second national quality control samples were detected. 2 nucleic acid extraction methods were used, 2 nucleic acid amplification test reagents were detected, and nucleic acids were extracted and amplified.Results The detection rates of OFR 1ab and N genes were 100.00% in the positive quality control of shuoshi automatic extractor + shuoshi nucleic acid detection reagent,The simultaneous detection rate of double genes was 100.00%, and the effective detection rate was 100.00%;In Shengxiang release agent + Shengxiang nucleic acid detection reagent group, The detection rate of OFR 1ab was 70.00%,The detection rate of N gene was 90.00%, the simultaneous detection rate of double gene was 70.00%, and the effective detection rate was 90.00%;In Shuoshi automatic extraction instrument + Shengxiang nucleic acid detection reagent group,The positive quality control OFR 1ab and N gene detection rate were 100.00%, double gene detection rate was 100.00%, the effective detection rate was 100.00%.Conclusion The positive quality control detection rate of shuoshi automatic extractor and its matching reagents, Shushi automatic extractor and Shengxiang nucleic acid detection reagent combination is higher than that of Shengxiang releaser + Shengxiang nucleic acid detection reagent combination, and the nucleic acid extraction efficiency of Shengxiang releaser needs to be improved. |
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| Keywords: | novel coronavirus interventricular quality assessment nucleic acid detection detection rate |
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