| 高效液相色谱法测定活血通络丸中紫丁香苷的含量 |
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| 引用本文： | 王刚,赵才德,马玉旬. 高效液相色谱法测定活血通络丸中紫丁香苷的含量[J]. 中国中医药信息杂志, 2009, 16(1): 54-55 |
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| 作者姓名： | 王刚 赵才德 马玉旬 |
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| 作者单位： | 1. 武威市药品检验所,甘肃,武威,733000
2. 武威市凉州区中医院,甘肃,武威,733000 |
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| 摘 要： | 目的建立活血通络丸中紫丁香苷含量的测定方法。方法采用高效液相色谱法，色谱柱为Kromasil C18（250mm×4.6mm，5μm），流动相为乙腈-1％冰醋酸（10：90），流速为1．0mL／min，检测波长为270nm，柱温为室温，进样量为20μL。结果紫丁香苷浓度在5．3～53μg／mL范围内线性关系良好，平均回收率为98．29％，RSD=1．71％。结论方法简便、准确、重现性好，可作为活血通络丸的质量控制方法。
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| 关 键 词： | 活血通络丸 高效液相色谱法 紫丁香苷 含量测定 |
Determination of Syringin in Huoxue Tongluo Pill by HPLC |
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| WANG Gang,ZHAO Cai-de,MA Yu-xun. Determination of Syringin in Huoxue Tongluo Pill by HPLC[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2009, 16(1): 54-55 |
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| Authors: | WANG Gang ZHAO Cai-de MA Yu-xun |
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| Affiliation: | WANG Gang, ZHAO Cai-de, MA Yu-xun (1. Wuwei Institute for Drug Control, Wuwei 733000, China： 2.Liangzhou TCM Hospital, Wuwei 733000, China) |
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| Abstract: | Objective To establish a HPLC method for the content determination of syringin in Huoxue Tongluo Pill. Methods Chromatographic assay was performed on Kromasil C18 column （250mm×4.6mm,5μm） at room temperature, and the mixture solution of acetonitrile-1% aqueous acetic acid （10 ： 90, v/v） was used as mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was at 270 nm. The sample size was 20 μL. Results Good linear relationship was achieved when the concentration of syringin was in the range of 5.3-53 μg/mL. The average recovery was 98.29% with RSD= 1.71%. Conclusion The method is simple, accurate and the results are stable with good reproducibility. This method can be used for the content determination of syringin in Huoxue Tongluo Pill. |
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| Keywords: | Huoxue Tongluo Pill HPLC syringin, content determination |
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