高纯度猪软骨Ⅱ型胶原修复兔膝关节软骨缺损的实验研究

目的 研制高纯度猪软骨Ⅱ型胶原海绵,探讨其修复关节软骨缺损的可行性.方法 将40只成年雄性新西兰兔制成膝关节软骨缺损模型,随机分成两组.A组20只兔的缺损区植入Ⅱ型胶原海绵,B组20只兔的缺损区旷置,不植入胶原作为对照.于术后2、4、8、12、24周分别做HE染色、Masson染色、Safranin O染色及Ⅱ犁胶原免疫组化检测.结果 ①HE染色及Masson染色显示A组在术后2周即出现新生软骨细胞,细胞以胶原纤维为支架迁移进入缺损区,并与胶原纤维生长融合;12周后新生骨和软骨组织完全填满缺损区并与周围组织整合,而B组直至术后24周仍由纤维组织所填充;②免疫组化检测结果 显示A组新生软骨细胞具有正常兔软骨细胞的表型;③Safranin O染色结果 显示A组新生软骨细胞具有分泌软骨基质的功能.结论 Ⅱ型胶原具有较强的诱导软骨细胞生长的能力,其诱导生长的新生软骨细胞具有正常透明软骨细胞的表型和功能,是良好的软骨缺损修复的生物填充材料.

The experimental study on the repair of articular cartilage defects of the knee in rabbits with type Ⅱ collagen

Objective To investigate the feasibility of repairing articular cartilage defects with type Ⅱ collagen prepared in our institute.Methods Forty adult male New Zealand white rabbits were used for preparing models of cartilage defects at the knee joint.The rabbits were randomly divided into 2 even groups. In Group A,the cartilage defects at the knee joint of 20 rabbits were filled with type collagen.In Group B, the cartilage defects at the knee joints of the other 20 rabbits were not treatedcontrols.The tissue samples of the kneejoint were collected and examined by H＆E,Safranin 0 and Masson staining and type Ⅱ collagen immunostaining at 2,4,8,12 and 24 weeks postoperatively. Results H＆E and Masson staining examinations revealed regeneration of cartilage in Group A 2 weeks postoperatively.The newborn ehondrocytes grew into the defect resion along the collagen fiber net.The new cartilage tissue filled up the whole defect region in Group A 12 weeks postoperatively,while only fibrous tissues could be found in the defect region in Group B 24 weeks postoperatively.Type Ⅱ collagen immunohistochemistry examination verified that the regenerated ehondrocytes exhibited a normal phenotype in Group A.The Safranin 0 examination also confirmed that the regenerated chondrocytes had a normal function of secreting cartilage matrix in Group A. Condusions Type Ⅱ collagen can induce regeneration of chondrecytes and promote healing of articular cartilage defects.The regenerated ehondrocytes induced by type Ⅱ collagen exhibit normal phenotype and function. Therefore,it may be a valuable stuffing biomaterial for repairing articular cartilage defects.