负压引流联合纳米银敷料促进感染切口愈合的实验及临床研究

目的探讨自制负压装置联合纳米银敷料促进大鼠切口感染愈合的优势,并探究其临床疗效。方法感染切口模型大鼠随机分为常规处理组、单纯负压吸引组、负压吸引联合银离子敷料组。治疗后评价各组大鼠切口愈合时间、愈合面积,对切口组织的炎症因子进行免疫组化及荧光定量分析。并将三组方法应用于临床手术部位感染患者,比较分析各组患者切口的肉芽覆盖时间、肉芽痊愈时间和切口愈合时间。结果大鼠肉芽组织免疫组织化学及IOD值、大鼠切口组织TNF-α、IL-2、IL-8的mRNA相对表达水平均低于常规处理组和单纯负压吸引组,差异有统计学差异(P0.05);临床应用中负压吸引联合银离子敷料组的切口愈合时间、术后C反应蛋白、疼痛评分等指标均优于常规处理组和单纯负压吸引组,差异有统计学差异(均P0.05)。结论与传统治疗手段相比,负压吸引联合银离子敷料治疗能更有效地控制手术部位感染,减轻切口局部炎症,促进切口愈合。

Experimental and clinical study on negative pressure suction combined with nano silver dressing for promoting healing of infected incision

ObjectiveTo evaluate the advantage of homemade negative pressure device combined with nano silver dressing for promoting the healing of infected incision in rats, and explore its clinical curative effect. MethodsInfected incision model rats were randomly divided into conventional treatment group, and simple pressure suction group, pressure suction combined with silver ion dressing group. The healing time and healing area of rats in each group after treatment were evaluated, immunohistochemical and fluorescent quantitative analysis of inflammatory factors in incisional wound tissue were performed. Three methods were applied to patients with surgical site infection(SSI), granulation coverage time, granulation recovery time, and incision healing time of three groups of patients were compared. ResultsImmunohistochemistry and its IOD value, the relative mRNA expression levels of TNF α, IL 2, and IL 8 in rat wound tissue treated with pressure suction combined with silver ion dressing were all inferior to conventional treatment group and simple negative pressure suction group, difference was statistically significant (P< 0.05); in clinical application, wound healing time, postoperative C reactive protein level, and pain assessment scores in patients treated with pressure suction combined with silver ion dressing were all superior to conventional treatment group and simple negative pressure suction group, difference were all statistically significant (all P< 0.05). ConclusionCompared with conventional treatment method, pressure suction with silver ions dressing treatment can more effectively control SSI, reduce local inflammation of incision, and promote incision healing.