五种常用消毒剂对金黄色葡萄球菌的杀灭效果评价

目的研究医院常用消毒剂乙醇、三氯片、碘伏、戊二醛、爱护佳免洗手消毒剂对医院不同来源金黄色葡萄球菌的杀灭效果,为有效控制医院感染提供科学依据。方法收集南昌大学第一附属医院临床送检标本和医护人员手及医院内周边环境物体表面采集的标本分离的金黄色葡萄球菌共48株。通过消毒剂与细菌直接接触作用,研究消毒剂在不同稀释浓度、不同作用时间、体外杀灭不同来源的金色葡萄球菌的效果。结果常规使用消毒剂如5 g/L碘伏、20 g/L戊二醛和爱护佳免洗手消毒剂(0.5%葡萄糖酸氯己定+70%乙醇)常温下作用5 min对不同来源金黄色葡萄球菌的杀灭率为100%;70%乙醇和1 g/L三氯片常温下作用5 min对不同来源金黄色葡萄球菌的杀灭率为96.5%~99.8%;但碘伏、戊二醛和免洗手消毒剂被高度稀释后即使提高作用时间但仍不能完全杀灭金黄色葡萄球菌。结论该院常规使用消毒剂能满足临床杀菌效果,但需要不断地监测消毒剂的有效浓度,防止杀菌能力降低。

Bactericidal efficacy of five kinds of disinfectant on Staphylococcus aureus

ObjectiveTo study the bactericidal efficacy of hospital commonly used disinfectants such as ethanol, 3" chlorine tablets, iodophor, glutaraldehyde, and avagard instant hand antiseptic on Staphylococcus aureus(S. aureus) from different sources of hospital, and provide scientific basis for effective control of healthcare associated infection(HAI).MethodsA total of 48 strains of S. aureus from inpatients, hands of health care workers, and environment surface in the First Affiliated Hospital of Nanchang University were collected. Disinfectant was directly contacted with bacteria, in vitro killing efficacy of disinfectant on S. aureus from different sources at different diluted concentrations, and different contact time were studied.ResultsThe killing rate of 5g/L iodophor, 20g/L glutaraldehyde, and avagard instant hand antiseptic(0.5% chlorhexidine+70% ethanol) to S. aureus with a 5 minute contact time was 100%; killing rates of 70% ethanol and 1g/L 3" chlorine tablets to S. aureus with a 5 minute contact time were 96.5%-99.8%;but highly diluted iodophor, glutaraldehyde, and avagard instant hand antiseptic still could not completely kill S. aureus even the contact time was extended.ConclusionThe routine use of disinfectants in the hospital can meet the clinical bactericidal efficacy, it is necessary to monitor concentration routinely, avoid decreasing sterilization ability.