核壳型Fe3O4@PDA@Au纳米材料对水牛角含巯基肽类成分的富集研究

目的?通过制备核壳型Fe₃O₄@PDA@Au纳米材料对水牛角中含巯基（-SH）肽类成分进行富集、鉴定与表征。方法?采用种子生长法制备核壳型Fe₃O₄@PDA@Au磁性纳米材料，将材料应用于水牛角提取液含-SH肽类成分的富集；分别采用Ellman法和多肽试剂盒测定富集前后溶液中-SH及多肽的含量；采用nano LC-MS/MS系统分析鉴定水牛角提取液肽类组成。结果?Fe₃O₄@PDA@Au纳米材料对水牛角含-SH肽类成分的富集因子为23.41，水牛角提取液中共鉴定了4?114个肽段，其中含有半胱氨酸（Cys）的肽段共11个；富集液中共鉴定了2?254个肽段，其中含有Cys的肽段共709个，富集后的含Cys的肽段与总肽段的比值从0.003增加到0.314，表明材料对水牛角提取物中含-SH肽段完成了较好的富集。结论?Fe₃O₄@PDA@Au纳米材料对水牛角中含巯基肽类成分具有良好的富集效果，对水牛角功效物质基础研究具有重要意义。 

Enrichment of Thiol-Containing Peptides in Water Buffalo Horn by Core-Shell Fe3O4@PDA@Au nano-Materials

OBJECTIVE?Enrichment， identification， and characterization of thiol-containing peptides in water buffalo horn by using core-shell Fe₃O₄@PDA@Au nano-material. METHODS?The core-shell Fe₃O₄@PDA@Au magnetic nano-materials were prepared by seed growth method， and the material was applied to the enrichment of thiol-containing peptides in water buffalo horn extract. The contents of -SH and peptide in water buffalo horn samples were determined by Ellman method and peptide assay kit， respectively. And then the peptides in water buffalo horn extract and enriched fraction were identified and analyzed by nano LC-MS/MS system. RESULTS?The enrichment factor of Fe₃O₄@PDA@Au nano-materials in the enrichment of thiol-containing peptides in water buffalo horn extract was calculated as 23.41. A total of 4114 peptides were identified in the water buffalo horn extract， including 11 peptides containing Cys residue; a total of 2254 peptides were identified in the enriched fraction， including 709 peptides containing Cys residue. After enrichment， the ratio of the number of thiol-containing peptide to the total peptide in water buffalo horn extract increased from 0.003 to 0.314. The results indicated that the nano-material had a high efficiency on the enrichment of thiol-containing peptides in water buffalo horn extract. CONCLUSION?Fe₃O₄@PDA@Au nano-materials can be used for the enrichment of thiol-containing peptides in water buffalo horn， which is of great significance for the basic research of water buffalo horn.