血培养报阳时间及病原菌分布

目的了解血培养阳性标本的菌群分布及报阳时间,为实验室病原菌诊断及临床制定治疗方案提供依据。方法收集2015年5—11月某院临床各科室送检的血标本,记录血培养报阳时间并将所获得病原菌鉴定至菌种。结果从血培养标本中共分离157株病原菌,其中革兰阳性球菌占31.85%,革兰阴性杆菌占57.32%,真菌占10.83%。培养阳性病原菌及其报阳的中位时间分别为:肠杆菌科细菌0.50 d,非发酵菌0.63 d,肠球菌属0.60 d,链球菌属0.80 d,葡萄球菌属1.01 d,真菌1.44 d。结论血培养阳性标本报阳时间从早到晚依次是:肠杆菌科细菌、肠球菌属、非发酵菌属、链球菌属、葡萄球菌属、真菌。引起血流感染的病原菌均在4 d内仪器阳性报警,大多数病原菌在1 d内报阳性。

Positive alarming time of blood culture and distribution of pathogens

ObjectiveTo investigate the distribution of pathogens and positive alarming time of blood culture, and provide basis for laboratory diagnosis and clinical treatment. MethodsBlood specimens from clinical departments in a hospital in May November 2015 were collected, positive alarming time of blood culture was recorded, species of pathogens were identified. ResultsA total of 157 pathogenic strains were isolated from blood culture specimens, gram positive cocci, gram negative bacilli, and fungi accounted for 31.85%, 57.32%, and 10.83% respectively. The median positive alarming time were as follows: Enterobacteriaceae 0.50 day, non fermenting bacteria 0.63 day, Enterococcus spp. 0.60 day, Streptococcus spp. 0.80 day, Staphylococcus spp. 1.01 days, and fungi 1.44 days, respectively. ConclusionPositive alarming time of blood culture specimens from early to late are as follows: Enterobacteriaceae, Enterococcus, non fermentative bacteria, Streptococcus spp., Staphylococcus spp., and fungus. Positive alarming time of pathogens causing bloodstream infection are all within 4 days, and most of them are within 1 day.