| 痢疾杆菌大质粒DNA的制备及影响因素 |
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| 引用本文： | 刘学博,孔祥英,毛培基,高杰英. 痢疾杆菌大质粒DNA的制备及影响因素[J]. 军事医学科学院院刊, 1988, 0(6) |
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| 作者姓名： | 刘学博 孔祥英 毛培基 高杰英 |
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| 作者单位： | 军事医学科学院微生物流行病研究所，军事医学科学院微生物流行病研究所，军事医学科学院微生物流行病研究所，军事医学科学院微生物流行病研究所 |
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| 摘 要： | 本实验采用碱变性的方法制备痢疾杆菌大质粒.在制备中观察了碱、温度、时间及SDS几个因素对质粒纯度的影响.诸因素中碱为关键因素,温度亦很重要.本文建立了小量检测及大量制备痢疾杆菌大质粒的方法并与文献中常用的超离心方法制备的大质粒做了电泳、酶切图谱的比较,其纯度不低于超离心纯化的质粒,这两种方法制备的质粒酶切图谱是一致的.说明本方法未使质粒DNA变性.
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| 关 键 词： | 痢疾杆菌 大质粒 染色体 氯化铯密度梯度超离心 |
PREPARATION OF LARGE PLASMID DNA OF SHIGELLA AND THE FACTORS AFFECTING IT |
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| Liu Xuebo,Kong Xiangying,Mao Peiji,Gao Jieying Institute of Microbiology and Epidemiology,Acadcmy of Military Medical Sciences. PREPARATION OF LARGE PLASMID DNA OF SHIGELLA AND THE FACTORS AFFECTING IT[J]. Bulletin of the Academy of Military Medical Sciences, 1988, 0(6) |
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| Authors: | Liu Xuebo Kong Xiangying Mao Peiji Gao Jieying Institute of Microbiology Epidemiology Acadcmy of Military Medical Sciences |
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| Abstract: | The effects of concentration of NaOH and SDS temperature and duration of incubation on the isolation and purification of large plasmid DNA of Shigella were studied.The concentration of alkaline was found to be critical and temperature to be important.The isolated plasmid DNA contained less chromosomal DNA and no RNA.It was digestable by restrictive enzymes without further purification.Results of electrophoretic assay of the products prepared by this method agreed quite well with that prepared by the classical ultra-centrifugation method. |
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| Keywords: | species Shigella chromasome large plasmid CsCl_2 density gradient centrifugation |
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