长远航持续应激对潜艇艇员免疫功能及细胞因子表达的影响

目的:研究某型潜艇艇员长远航前后淋巴细胞亚群、调节T淋巴细胞及相关细胞因子的变化,探讨长远航中持续应激对艇员免疫功能的影响。方法:随机抽取长远航(70 d)潜艇61名艇员为研究对象,空腹抽取静脉血,利用流式细胞仪对艇员长远航前后淋巴细胞亚群[CD3 +、CD4 +、CD8 +、CD4 +/CD8 +、CD3 -CD19 +、CD3 -CD(16+56) +]及调节T淋巴细胞(CD4 +CD25 +)进行测定,采用液态芯片技术观察白细胞介素-2(IL-2)、IL-4、IL-8、干扰素诱导蛋白-10(IP-10)含量的变化。 结果:与长远航前相比,长远航后CD3 +、CD4 +、CD8 +、CD3 -CD19 +、CD3 -CD(16+56) +淋巴细胞水平显著降低,差异均有统计学意义( P<0.05);CD4 +/CD8 +的比值以及CD4 +CD25 +水平显著升高,差异均有统计学意义( P<0.01);IL-2、IL-8含量降低,差异均有统计学意义( P<0.05);IL-4、IP-10含量升高,差异均有统计学意义( P<0.05)。 结论:在长远航持续应激作用下,艇员的免疫功能发生明显变化,表明长远航对艇员健康有一定的影响,有必要进一步阐明原因并采取必要的预防措施。

Effects of persistent stress of long oceangoing voyage on the immune function and cytokine expression in submarine crew

Objective To study the changes of the lymphocyte subpopulation,regulatory T cells,and related cytokines before and after long oceangoing voyage,and to explore the effect of persistent stress on immune function in submarine crew.Methods Sixty-one submariners of long oceangoing voyage(70 d)were selected randomly.Before and after the voyage,the quantities of lymphocyte subpopulations[CD3+,CD4+,CD8+,CD4+/CD8+,CD3-CD19+,CD3-CD(16+56)+]and regulatory T cells(CD4+CD25+)from peripheral blood were detected by flow cytometry;and the contents of interleukin-2(IL-2),interleukin-14(IL-14),interleukin-8(IL-8),and interferon inducible protein 10(IP-10)were detected and compared by liquid chip.Results Compared with those before voyage,the levels of CD3+,CD4+/CD8+,CD3-CD19+,CD3-CD(16+56)+decreased significantly(P<0.05),CD4+/CD8+and CD4+CD25+increased significantly(P<0.01).The contents of IL-2 and IL-8 were lower markedly than those before voyage(P<0.05),while the contents of IL-4 andIP-10 were higher than those before voyage(P<0.05).Conclusion The immune function of submarine crew had some obvious changes under persistent stress of long oceangoing voyage,indicating long oceangoing voyage had certain effects on the health of submarine crew;which is necessary to make further research on the mechanism and take preventive measures.