| 高效液相色谱法测定清热解毒片中哈巴俄苷的含量 |
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| 引用本文： | 刁红雨,;王维礼,;程晓英.高效液相色谱法测定清热解毒片中哈巴俄苷的含量[J].中国中医药杂志,2008,6(7):9-11. |
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| 作者姓名： | 刁红雨 ;王维礼 ;程晓英 |
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| 作者单位： | [1]吉林省医疗器械检验所,130062; [2]吉林省吉林市食品药品检验所,132001 |
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| 摘 要： | 目的：建立清热解毒片的含量测定方法。方法：采用高效液相色谱法。色谱柱：SHIMADZU VP-ODS250L×4．6，流动相：乙腈-1％醋酸溶液（0～30分种，20→40：80→60，V／V），流速：1．0ml·min^-1，检测波长：278mm，柱温：40℃。结果：哈巴俄苷在0．1～0．3mg·ml^-1范围内线性关系良好（r=1．0000）；平均加样回收率为99．21%。RSD=0．7％（n=6）。结论：该方法简便易行，结果准确可靠。可适用于清热解毒片的质量控制。
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| 关 键 词： | 高效液相色谱法 清热解毒片 哈巴俄苷 含量测定 |
Determination of Harpagoside in Qingrejiedu Tablets by HPLC |
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| Institution: | Diao Hong-yu Wang Wei-li Cheng Xiao-ying (Jilin Institute for Medical Appliance Control Jilin Changchun 130662 ; 2Jilin Institute for Food and Drug Control Jilin Jilin 132001) |
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| Abstract: | Objective：To set up a method for quality control of Qingrejiedu Tablets. Method：HPLC Method was developed to quantitative determination. COLUMN SHIMADZU VP-O0DS 250L×4. 6. The mobilic phase was acetonitrile-1% acetic acid solution（0-30minute20→40：80→60V/V）. The column temperature was 40 ℃, the wavelength for detection was 278nm, flow rate was 1. 0ml · min^-1.Results：The Harpagoside average of recovery rate was 99.21%,and RSD was 0.7%（n=6）.Conclusion：The method is simple, accurate and suitable for its assaying. |
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| Keywords: | HPLC Qingrejiedu Tablets Harpagoside Detemination |
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