人乳头瘤病毒16、18与宫颈癌及宫颈上皮内瘤变关系的研究

目的研究人乳头瘤病毒(HPV)16、18型感染与宫颈癌及宫颈上皮内瘤变(CIN)的关系。方法HPV(16、18型)核酸扩增(PCR)荧光检测试剂盒对118例患者宫颈组织中HPV16、18DNA进行检测,其中鳞癌(SCC)37例、腺癌(AUC)16例、CIN 45例(Ⅰ、Ⅱ、Ⅲ级各15例)及正常宫颈组织(NC)20例。结果(1)在SCC中HPVl6阳性率为75.68%,明显高于CIN(35.56%)、AUC(25%)及NC(5%)(P<0.01,P<0.01,P<0.01);CIN中HPV16阳性率明显高于NC(P<0.01);(2)AUC中HPV18阳性率为62.50%,明显高于SCC(8.11%)、CIN(4.44%)、及NC(0.00%)(P<0.01,P<0.01,P<0.01);(3)CINⅡ、Ⅲ级HPV16阳性率明显高于CIN I级(P<0.01,P<0.05);(4)不同分化SCC、AUC相互间HPVl6、18阳性率比较差异无显著性。结论(1)SCC的发生可能和HPVl6感染有关而AUC发生可能和HPVl8感染有关;(2)HPV16感染可能在CINⅠ发展到CINⅢ的过程中起重要作用;(3)SCC、AUC的细胞分化程度和HPV16、18感染无明显关系。

Studies on relationships between human Papillomavirus(HPV)16,18 and cervical cancer as well as cervical intraepithelial neoplasia

Objective The aim of this study was to investigate the relationships between human papillomavirus(HPV) 16,18 and cervical cancer as well as cervical intraepithelial neoplasia.Methods HPV 16,18 DNA in the tissue samples of 37 cases of squamous carcinoma of the cervix(SCC),16 cases of adenocarcionma of uterine cervix(AUC),45cases of cervical intraepithelial neoplasia(CIN),20 cases of normal cervix(NC) were detected by fluorescence polymerase chain eactin(PCR) diagnostic kit.Results (1) In SCC,The positive rate of HPV16(75.68%) was significantly higher than those in AUC(25%) and CIN was higher than NC(P<0.01).(2) In AUC,the positive rate of HPV18((62.50%)) was higher than those in SCC(8.33%) and CIN(4.44%),as well as NC(0.00%)(P<(0.01,) P<0.01,P<0.01).(3) In CINII and CINIII,the positive rate of HPV16 was higher than CINI(P<0.05,P<0.05).(4) No significant difference can be found in the different differentiation cervical carcinoma in respect to the positive rate of HPV 16,18.Conclusion(1) The oncogenesis of SCC might be associated with HPV16 infection,while the oncogenesis of AUC might be associated with HPV18.(2) HPV16 infection may plays important role in the development from CINI to CINIII.(3) There are no apparent relationships between the differentiating degrees of SCC,AUC and HPV16,18 infection.