猴病毒40T抗原和P53蛋白在毛丝鼠中耳上皮细胞系的表达及其意义

目的　证实猴病毒 40 (simianvirus40 ,SV40 )T抗原 (largeT antigen ,TAg)和P5 3蛋白在毛丝鼠 (chinchilla)中耳上皮细胞系细胞核内的表达 ,初步探讨它们在细胞无限增殖 (immortalization)过程中的作用。方法　应用免疫荧光组织化学原理 ,分别检测SV40TAg和P5 3蛋白在培养液内注入腺病毒 12 猴病毒 40混合病毒 (实验组 )及未在培养液内注入上述病毒 (对照组 )的体外培养毛丝鼠中耳上皮细胞核内的表达。结果　实验组的 8个培养皿中 ,1个培养皿内的细胞核内表达SV40TAg和P5 3蛋白 ,且已持续培养 46代以上 ,形成细胞系 ;其余 7个培养皿内的细胞和对照组细胞核内均不表达SV40TAg和P5 3蛋白 ,且培养到 6～ 8代时均自行凋亡。结论　SV40TAg和P5 3蛋白在细胞无限增殖过程中起重要的作用 ;它们在细胞核内的表达对毛丝鼠中耳上皮细胞系的建立可能具有密切的相关性。

Expressionof simian virus 40 large T-antigen and p53 protein in chinchilla middle ear epithelialcell line and its significance

Objective To identify the expression of simian virus 40 large T antigen (SV40TAg) and p53 protein in the cell nuclei of chinchilla middle ear epithelial cell line and explore the significance of the expression. Method To examine the expression of SV40TAg and p53 protein in cell nuclei of chinchilla middle ear epithelial cell which had been infected and had not been infected with Ad 12 SV40 hybrid virus by immunocytochemistry method. Results All of the cell nuclei in 1 of 8 culturing dishes which infected with the Ad12 SV40 virus expressed the SV40TAg and p53 protein and had cultured over 46 passages. They have become a cell line. All of the cells in rest 7 culturing dishes and the control cells did not express the SV40TAg and p53 protein in their nuclei and died after culturing 6 8 passages. Conclusion The SV40TAg and p53 protein play some important role in process of cell immortalization. The expression of SV40TAg and p53 protein in cell nuclei of CMEE 1 cell line has close interrelations with establishing the CMEE 1 cell line.