microRNA-224通过调节Bim影响人非小细胞肺癌的增殖与凋亡

目的:研究microRNA-224(miR-224)在人非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达及对NSCLC细胞增殖能力和凋亡的影响?方法:采用荧光实时定量PCR(qPCR)检测20对NSCLC组织与癌旁组织中miR-224的表达量,以及miR-224在NSCLC细胞A549与正常肺支气管上皮细胞中的表达量,并计算差值;转染anti-miR-224至A549细胞,qPCR检测anti-miR-224的转染效率;四甲基偶氮唑盐(MTT)实验及克隆形成实验检测miR-224对A549细胞增殖能力的影响,流式细胞技术检测细胞周期及凋亡;荧光素酶报告实验证实miR-224与靶基因Bim的结合;qPCR及Western blot检测转染anti-miR-224后A549细胞中Bim的表达量?结果:与癌旁正常组织相比,miR-224在NSCLC组织及细胞中均显著高表达(P < 0.05);抑制miR-224能够显著抑制A549细胞的增殖能力并促进凋亡,并促进了Bim的表达?结论:miR-224在NSCLC中呈高表达,miR-224能够通过调控Bim抑制A549细胞的增殖能力并诱导凋亡?

MicroRNA-224 effcet proliferation and apoptosis of NSCLC cell via downregulation of Bim

Objective:To explore the expression of microRNA-224 (miR-224)in non-small cell lung cancer (NSCLC)and its effect proliferation and apoptosis of NSCLC cell. Methods:Real-time quantitative PCR(qPCR)was used to detect and compare the expression of miR-224 in 20 pairs of NSCLC,adjacent normal tissue,NSCLC cell line A549 and normal epithelium cell line 16HBE. The expression of miR-224 in A549 cell was down-regulated by anti-miR-224 transient transfection,and the effect was identified by qPCR. The influence of miR-224 on A549 cell proliferation was detected by MTT assay and colony formation assay. Flow cytometry was used to test cell cycle and apoptosis. The expression of Bim was tested by anti-miR-224 transient transfection,and the effect was identified by qPCR and western blot. Results:Compared with adjacent normal tissues,miR-224 were significantly up-regulated in NSCLC tissue and cell.(P < 0.05). Down-regulating miR-224 can inhabits proliferation and accelerates apoptpsis as well as increasing the expression of Bim. Conclusion:The expression of miR-224 is up-regulated in NSCLC. MiR-224 can promotes proliferation and inhabits apoptosis by down-regulating Bim.