格列本脲改善小鼠局灶性脑缺血再灌注损伤后期的神经运动功能障碍

目的:研究格列本脲对脑缺血再灌注损伤小鼠后期神经运动功能障碍的影响?方法:应用C57BL/6小鼠,制备局灶性脑缺血再灌注损伤模型,经灌胃给予格列本脲(20 mg/kg)连续治疗5周,每周监测小鼠的空腹血糖与体重变化?通过角测试?圆柱体测试?转棒实验及踏空实验等观察小鼠的行为学改变,免疫组织化学染色法观察缺血脑区星形胶质细胞的变化?结果:格列本脲(20 mg/kg)连续治疗5周,不影响小鼠的空腹血糖;与对照组比较,从治疗第2周开始,能显著增加小鼠的体重(P < 0.01);从治疗第3周开始,能减少踏空实验中小鼠的足失误率(P < 0.05)及延长转棒实验中的棒上停留时间(P < 0.05),并减少缺血脑区胶质瘢痕的形成范围(P < 0.05)?结论:格列本脲连续治疗能够促进小鼠脑缺血再灌注损伤后期神经运动功能的恢复?

Glibenclamide improves long-term neurological deficits of cerebral ischemia/reperfusion injury in mice

Objective:To study the effects of glibenclamide on long-term neurological deficits of cerebral ischemia/reperfusion injury in mice. Methods:C57BL/6 mice were performed to establish the cerebral ischemia/reperfusion injury models by occluding the right middle cerebral artery (MCAo). Then,we continuously treated them with glibenclamide (20 mg/kg)by intragastric administration for 5 weeks. We monitored the fasting blood sugar (FBS)and weight of mice per week. We observed the neurobehavioral changes by behavior tests including corner test,cylinder test,rotarod test and foot fault test. Moreover,we observed the changes of astrocytes by immunohistochemistry staining. Results:Continuous treatment with glibenclamide for 5 weeks had no effect on the FBS. Compared to the control group,it significantly increased the body weight at the 2nd week of treatment(P < 0.01),and at the 3rd week of treatment,it reduced the foot fault rate (P < 0.05)and prolonged the time of animals remained on the rotating rod (P < 0.05). In addition,it reduced the area of glial scar (P < 0.05). Conclusion:Continuous treatment with glibenclamide can advance long-term functional recovery of cerebral ischemia/reperfusion injury in mice.