脱氧雪腐镰刀菌烯醇生物可及性体外消化模型的构建及验证

目的构建脱氧雪腐镰刀菌烯醇(DON)生物可及性体外消化模型并验证,并对影响模型的主要因素进行优化。方法模拟胃肠道生理消化过程,研究消化时间、禁食和进食状态、消化液体积、消化液pH值、同时进食的多种食物成分等对DON在口腔、胃和小肠中生物可及性的影响,确定DON生物可及性体外消化模型最优技术参数,并对模型进行验证。结果经过优化,食品中DON释放到唾液、胃液、十二指肠液和胆汁中浓度达最高的最佳条件为:口腔、胃和十二指肠中的消化时间分别为6min、1.5h和1.5h;消化液pH分别为6.5、0.9、7.5和8.0;消化液体积分别为6、13、11ml和6ml;禁食和进食等各种状态下DON生物可及性范围为81.28%～99.58%,DON的生物可及性随胃内容物的增加而降低;食品基质类别(玉米和小麦粉)对DON生物可及性无影响;同时进食的其他食物成分可降低谷物中DON的生物可及性。结论构建的DON体外消化模型稳定性高、重复性好,完全可以满足对食品中DON生物可及性的研究。

Development of in vitro digestion model for assessing the bioaccessibility of deoxynivalenol derived from foods

Objective To develop and validate in vitro digestion model for assessing the bioaccessibility of deoxynivalenol(DON) derived from foods,and optimize the major parameters affecting the model.Method To study the effect of digestion time,the fasting and feeding status,the volume and the pH of digestive juice,as well as other food components ingested with DON derived from mixed foodstuff on the bioaccessiblity of DON in a model simulating the process manner on gastrointestinal physiological digestion.To optimize some technical parameters of the model and validate it with both mycotoxin adsorbents and foods naturally contaminated by DON.Results The optimized conditions of which DON released from the ingested foods at the highest concentration in gastrointestinal tract are as follows: digestion of 6 min,1.5h and 1.5h in mouth,stomach and duodenum,respectively.At pH value of 6.5,0.9,7.5 and 8.0 as well as digestive juice volume of 6 ml,13 ml,11 ml and 6 ml for saliva,gastric juice,intestinal juice and bile,respectively.The bioaccessibility of DON at fasting and different feeding status was in the range from 81.28% to 99.58%,and the bioaccessibility of DON was reduced with the increase of contents in stomach.No significant effect of food matrix type(corn or wheat) on the bioaccessibility of DON was observed.However,other food components consumed along with DON can reduce the bioaccessibility of DON in cereals.Conclusion The in vitro digestion model developed for assessing the bioaccessibility of DON is stable and reproducible,which can meet the requirement of studies on the bioaccessibility of DON derived from foods.