Optimization of pulsed-field gel electrophoresis protocols for Salmonella Paratyphi A subtyping |
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| Authors: | Chen Chunxia Zhao Yingwei Han Hui Pang Bo Zhang Jingyun Yan Meiying Diao Baowei Cui Zhigang Zhou Haijian Liang Weili Feng Yanfang Kan Biao |
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| Affiliation: | State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 155 Changbai Road, Beijing, China. |
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| Abstract: | Salmonella enterica serovar Paratyphi A infection has caused public health problems in some countries in recent years. Pulsed-field gel electrophoresis (PFGE) has been used for the subtyping and epidemiological investigations of some serotypes of Salmonella, mainly in outbreaks caused by non-typhoidal Salmonella. In this study, different restriction endonucleases and electrophoresis parameters were compared for the PFGE subtyping by using Salmonella Paratyphi A strain panels. Two protocols for the enzymes SpeI and XbaI showed higher discriminatory power, which may facilitate epidemiological analysis for more accurate case definition, and clonality study of Salmonella Paratyphi A. |
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