宫颈鳞癌HPV16感染及其与c-myc p53表达的关系

目的：探讨HPV16在宫颈鳞癌发生发展中的作用及其与c-myc、p53表达产物之间的关系。方法：采用原位杂交和免疫组化方法检测了18例慢性宫颈炎、28例CIN、4例宫颈浸润性腺鳞癌和55例宫颈浸润性鳞癌HPV16 E6 DNA及其蛋白和c-myc、p53蛋白的表达情况。结果：浸润性宫颈癌。HPV16 E6 DNA及其蛋白、p53蛋白阳性率明显高于慢性宫颈炎组，c-myc蛋白阳性率则明显低于慢性宫颈炎组。p53蛋白阳性率与HPV16 E6 DNA的检出率之间无负相关关系。结论：宫颈鳞癌的发生发展可能与HPV16 E6 DNA的检出及c-myc、p53蛋白的表达异常相关。

STUDY OF HPV16 INFECTION IN CERVICAL SQUAMOUS CANCINOMA AND THE RELATION TO THE EXPRESSION OF C-MYC AND p53 PROTEIN

Objective:To study the role of HPV16infection in the carcinogenesis of cervical squa-mous carcinoma and the relationship between HPV16infection and the expression of c - myc,p53protein in cervical squamous carcinoma.Methods:In Situ hybridization and immunohistochemical method were used to detect HPV16E6DNA,E6protein,c - myc and p53protein in18cases of chronic cervicitis,28cases of cervical intraepithelial neoplasia and59cases of invasive cervical carcinoma.Results:A higher detection rate of HPV16E6DNA,E6protein and p53protein were found in invasive cervical carcinoma which c - myc positive expression rate was significantly lower than chronic cervicitis.Conclusion:Carcinogenesis of cervi-cal squamous carcinoma may be related to the presence of HPV16E6DNA and the abnormal expression of c - myc,p53protein.