胸腹气街的形态学基础

目的 :探求俞、募穴与其相应脏腑之间的神经联系通路。方法 :选取大鼠的 1 0对俞募穴及 4个非穴点为实验用穴 ,应用荧光双标记法进行研究。结果 :各俞募穴组及与脏腑位于同一神经节段的非穴点组分别在脊神经节内出现双标细胞。结论 :①脊神经节中存在可双重支配脏腑和体表的神经元 ,不仅使牵涉痛的机制得到进一步的解释 ,而且揭示了针刺穴位的感觉冲动是通过分支的传入轴突影响到内脏的功能和感觉或对某些脏器的活动产生影响。②为临床应用俞募穴治疗脏腑病提供了形态学理论根据。③进而揭示中医经络学中气街的现代生物学本质。

Morphological Basis of "the Pathway of Qi"

Objective:To explore the neural pathway between th e internal organs and their B ack-Shu and Front-Mu points. Methods: In 170 Wistar rats, fluorescent double-lab eling method was used in this study. The tracer agents 10% Propidum iodide (PI, 20 μL) and 10% Bisbenzimide (Bb, 20 μL) were separately injected into “Gansh u” (BL 18), “Qimen”(LR 14) and liver, “Xinshu”(BL 15), “Juque”(CV 14) and hea rt, etc.. The labeled cells in the related dorsal root ganglia (DRG) were detect ed under microscope. Results: Regarding Zang- organs, the doubly labeled neurons of “Ganshu”(BL 18) and liver were found from T 8 to T 12, those of “Q imen”(LR 14) and liver: T 8～T 12; “Ganshu”, “Qimen”: T 7～T 11; “Xinsh u”(BL 15) and hear t: T 3～T 6; “Juque”(CV 14) and heart: T 3～T 7; “Xinshu” and “Juque”: T 2～T 6; “ Pishu”(BL 20) and spleen: T 10～L 1; “Zhangmen”(LR 13) and spleen: T 10～T 13; “Pi shu” and “Zhangmen”: T 9～L 1; “Feishu”(BL 13) and lung: C 8～T 5; “Zh ongfu”(LU 1) and lung: T 1～T 5; “Feishu” and “Zhongfu”: C 8～T 4; “Shenshu”(BL 23) and ki dney: T 13～L 3; “Jingmen”(GB 25) and kidney: T 12～L 2; “Shenshu ” and “Jingmen” : T 13～L 1. In regard to Fu-organs, the doubly labele d neu rons of “Danshu”(BL 19) and common bile duct were found from T 9 to T 12 ; “Riyue”(GB 24) and common bile duct: T 10～T 11; “Danshu” and “Riyue”: T 9～T 11; “We ishu”(BL 21) and stoma ch: T 9～T 13; “Zhongwan” and stomach: T 8～T 12; “Weishu” and “ Zhongwan”: T 9～T 12; “Dachangshu”(BL 25) and large intestine: L 2～L 5; “Tianshu”(ST 25) and lar ge intestine: T 12～L 4; “Dachangshu” and “Tianshu”: T 13～L 2; “ Xiaochangshu”(BL 2 7) and small intestine: L 1～L 4; “Guanyuan”(CV 4) and small intestine: T 13～L 3; “Xiaochangshu” and “Guanyuan”: L 1～L 3; “Pangguangshu”(BL 28) and bladd er: L 5～S 2; “Zhongji”(CV 3) and bladder: L 5～S 3; “Pangguangshu” and “Zhon gji”: L 4～S 2. Conclusion: There exist neurons in the spinal DRG that contr ol the internal or gans and the body surface, which not only provides reasonable explanations about the mechanism of the referred pain, but also suggests that the sensory impulses of acupuncture stimulation may exert a direct effect on the functional activiti es of the internal organs through the branches of the afferent nerve. Results of the present study provide anatomical basis for the theory of “Qijie” (pathway of qi) and clinical application of Back-Shu- and Front-Mu-poi nts in the treatment of visceral disorders.