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HLA-B*15 subtypes in Burmese population by sequence-based typing |
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| Authors: | C Kongmaroeng A Romphruk R Ruangwerayut C Paupairoj C Leelayuwat H Inoko & A Romphruk |
| Institution: | PhD Program in Biomedical Science, Faculty of Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand; The Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Clinical Immunology and Transfusion Medicine, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand; Mae Sot Hospital, Mae Sot, Tak, Thailand; Blood Transfusion Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand; Department of Genetic Information, Division of Molecular Life Science, Tokai University, Japan |
| Abstract: | Human leukocyte antigen (HLA)-B*15 encompasses an increasing number of subtypes of more than 150. Frequency studies and a strong genetic association between HLA subtypes and susceptibility to drug hypersensitivity have been reported in different ethnic populations. To identify HLA-B*15 subtypes in Burmese using sequence-based typing (SBT) method, we selected 65 HLA-B*15 -positive samples from 170 unrelated healthy Burmese who were genotyped HLA-B * by polymerase chain reaction with the sequence-specific primer method. The frequency of HLA-B*15 in Burmese was found to be 38.2%. By the SBT method, results showed 10 alleles of HLA-B*15 subtypes. Four common alleles, B*1502 (45.2%), B*1532 (16.4%), B*1525 (12.3%), and B*1501 (8.2%), were found in 82.1% of HLA-B*15 -positive Burmese. Whereas the B*1501 was the highest in the Caucasians, Koreans, and Japanese, the highest frequency of HLA-B*15 alleles in Burmese was B*1502 (45.2%) that is similar to the frequency found in northeastern Thais and Vietnamese. This study is the first report of HLA-B*15 subtypes in Burmese. These results will provide the basic data in the further study in transplantations, genetic association with diseases, and drug hypersensitivity. |
| Keywords: | Burmese HLA-B*15 subtypes sequence-based typing |