自体富血小板血浆联合低分子肝素对吸入性损伤大鼠治疗效果的研究

目的：探讨自体富血小板血浆（ PRP ）联合低分子肝素（ LMWH ）治疗吸入性损伤大鼠的治疗效果。方法选取SPF级健康雄性Wistar大鼠48只，随机分为正常组、模型组、单用组、联合组，每组12只，模型组、单用组和联合组均建立烟雾吸入性损伤大鼠模型，模型组、正常组气管内滴入0乔．9％氯化钠溶液，单用组致伤后气管内滴注PRP液、联合组致伤后气管内滴注PRP和LMWH混合液。结果模型组、单用组、联合组的肺组织含水量显著的高于正常组（ P ＜0．05），联合组的肺组织含水量显著的低于模型组和单用组（ P ＜0．05）；模型组、单用组、联合组的血清TNF-α、IL-6、IL-1β、PDGF显著高于正常组（ P ＜0．05），联合组的血清TNF-α、IL-6、IL-1β、PDGF显著的低于模型组和单用组（ P ＜0．05）；模型组、单用组、联合组的肺组织Brdu免疫组化阳性标记指数显著的高于正常组（ P ＜0．05），联合组的肺组织Brdu免疫组化阳性标记指数显著的高于模型组和单用组（ P ＜0．05）。结论 PRP联合LMWH治疗吸入性损伤大鼠能够显著的减轻肺水肿及炎性反应。

Experimental study on the therapeutic effects of autologous platelet rich plasma combined with low molecular weight heparin on inhalation pulmonary injury in rats

Objective To investigate the therapeutic effects of autologous platelet rich plasma ( PRP) combined with low molecular weight heparin ( LMWH) on inhalation pulmonary injury in rats.Methods Forty-eight healthy rats ( SPF grade) were randomly divided into control group, model group, with 12 rats in each group.The animal models with smoke inhalation pulmonary injury were established in rats of model group, singleu-se group and combination group,and the rats in model group and control group were given 0.9% sodium chloride solution by intratracheal instillation , however,after smoke inhalation pulmonary injury the rats in single-use group were given PRP liquor by intratracheal instillation , but the rats in combinationgroup were given mixed liquor of PRP with LMWH by intratracheal instillation .Rseults The water content of lung tissues in model group, single-use group, combination group was significantly higher than that in control group ( P <0.05),moreover, which in combination group was significantly lower than that in model group and single-use group ( P <0.05).The serum levels of TNF-α,IL-6,IL-1β,PDGF in model group,single-use group, combination group were significantly higher than those in control group ( P <0.05),however, which in combination group were significantly lower than those in model group and single-use group ( P <0.05).The Brdu positive immunohistochemical labeling indexes in model group, singleu-se group, combination group were significantly higher than those in control group ( P <0.05), furthermore, which in combination group were significantly higher than those in model group and single -use group (P <0.05).Conclusion PRP combined with LMWH can obviously alleviate pulmonary edema and inflammation reaction in treatment of inhalation pulmonary injury in rats.