16S rRNA序列测定结肠腺瘤性息肉与肠道菌群的相关性分析

目的探讨作为结肠癌癌前病变的结肠腺瘤性息肉(CAP)中肠道菌群的改变特征。方法随机收集2017年11月至2018年4月在昆明医科大学第一附属医院进行结肠镜检查的结肠腺瘤性息肉患者(CAP组)30例和无腺瘤息肉的健康个体(HC组)30名。通过内镜下电凝电切治疗,收集CAP患者的腺瘤组织和健康志愿者(HC)的肠黏膜组织,提取基因组DNA,对细菌16S rRNA基因V3~V4区进行扩增,通过Illumina MiSeq平台进行序列测定,实验结果使用秩和检验(Wilcoxon检验)进行比较法分析。结果CAP患者的腺瘤组织中肠道菌群α多样性高于健康肠黏膜组织(Chao指数、Ace指数P<0.01)。门水平分析中,CAP组中拟杆菌门(FC=0.38)的丰度显著降低(P<0.01);属水平分析中,拟杆菌属(FC=0.32)、埃希菌属(FC=0.57)、瘤胃球菌属(FC=0.42)、Blautia(FC=0.27)、Dorea(FC=0.57)在CAP组中丰度降低(P<0.05);假单胞菌属(FC=2.43)、乳球菌(FC=2.84)、土芽孢杆菌属(FC=2.07)和不动杆菌属(FC=2.36)丰度升高(P<0.05)。结论与HC肠黏膜组织相比,CAP患者腺瘤组织中菌群的丰度和多样性存在显著的差异,表明腺瘤性息肉患者黏膜中菌群存在失衡现象。这种肠道微环境的失衡,对研究结肠腺瘤性息肉的发生和发展具有重要意义。

Analysis of gut microbiota change in colorectal adenomatous polyps patients by 16S rRNA sequencing

Objective To explore the characteristics of gut microbiota change in colorectal adenomatous polyps(CAP),which has been considered as precancerous lesion for colorectal cancer.Methods Thirty patients with colon adenomatous polyps(CAP group)and thirty healthy individuals without adenomatous polyps(HC group)who underwent colonoscopy at the First Affiliated Hospital of Kunming Medical University from November 2017 to April 2018 were randomly collected.The biopsy mucosae were collected by endoscopic electrocoagulation,and DNA was extracted to amplify 16S rRNA V3-V4 region,followed high-throughput sequencing with Illumina MiSeq platform.The experimental results were analyzed using Wilcoxon test.Results The alpha diversity of CAP patients was higher than that of healthy controls(Chao&Ace P<0.01).A decreased abundance of Bacteroidetes(FC=0.38)was observed at phylum level(P<0.05).At genus level,the abundances of Bacteroides(FC=0.32),Escherichia(FC=0.57),Ruminococcus(FC=0.42),Blautia(FC=0.27),and Dorea(FC=0.57)were decreased(P<0.05),but those of Pseudomonas(FC=2.43),Lactococcus(FC=2.84),Geobacillus(FC=2.07),and Acinetibacter(FC=2.36)were increased in CAP patients(P<0.05).Conclusions Compared with healthy volunteers,there are significant differences in the abundance and diversity of the adenoma tissue in CAP patients,indicating that there is an imbalance of gut microbiota in the adenomatous polyps.The imbalance of intestinal microenvironment may contribute to the occurrence and development of CAP.