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| 中等强度冲击波负压暴露对豚鼠耳蜗毛细胞游离钙浓度的影响 | |
| 引用本文： | 李朝军,刘兆华,朱佩芳,王正国,刘宝松,杨成,陈海斌,宁心. 中等强度冲击波负压暴露对豚鼠耳蜗毛细胞游离钙浓度的影响[J]. 西南国防医药, 2006, 16(1): 20-22 |
| 作者姓名： | 李朝军 刘兆华 朱佩芳 王正国 刘宝松 杨成 陈海斌 宁心 |
| 作者单位： | 1. 第三军医大学大坪医院野战外科研究所耳鼻咽喉头颈外科,重庆,400042 2. 第三军医大学大坪医院野战外科研究所第四研究室 3. 第三军医大学大坪医院野战外科研究所第三研究室 |
| 基金项目： | 致谢：激光共聚焦显微镜观察和数据分析,得到第三军医大学基础部中心实验室热情帮助,特此致谢! |
| 摘 要： | 目的:观察实验性冲击波负压(BUP)暴露对豚鼠耳蜗外毛细胞内游离钙浓度([Ca2 ]i)的影响。方法:在激光共聚焦显微镜下,用钙敏荧光探针Fluo-3作为指示剂,观察豚鼠暴露中等强度实验性BUP后耳蜗外毛细胞[Ca2 ]i的变化。结果:中等强度BUP暴露后8 h,至暴露后24 h和3 d稍有回落,但仍高于正常对照组。上述这些变化与听性脑干反应阈值的升高是一致的。结论:豚鼠暴露中等强度性BUP可引起耳蜗外毛细胞内[Ca2 ]i的明显增高,且可能是听功能损害的主要原因之一。 |
| 关 键 词： | 冲击波负压 创伤 游离钙离子 毛细胞 豚鼠 |
| 文章编号： | 1004-0188（2006）01-0020-03 |
| 收稿时间： | 2005-09-26 |
| 修稿时间： | 2005-09-26 |
Effects of exposure to moderate blast underpressure on calcium ion of cochlea hair cells in guinea pig |
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| LI Chao-jun,LIU Zhao-hua,ZHU Pei-fang,WANG Zheng-guo,LIU Bao-song,YANG Cheng,CHEN Hai-bin,NING Xin. Effects of exposure to moderate blast underpressure on calcium ion of cochlea hair cells in guinea pig[J]. Medical Journal of National Defending forces in Southwest China, 2006, 16(1): 20-22 | |
| Authors: | LI Chao-jun LIU Zhao-hua ZHU Pei-fang WANG Zheng-guo LIU Bao-song YANG Cheng CHEN Hai-bin NING Xin |
| Affiliation: | 1. Department of Otolarynogoey, Head and Neck Surgery, Surgery Institute of Daping Hospital, Third Military Medical University, Chongqing, 400042; 2.Fourth Department of Research, Surgery Institute of Daping Hospital, Third Military Medical University, Chongqing, 400042; 3. Third Department of Research, Surgery Institute of Daping Hospital, Third Military Medical University, Chongqing, 400042 |
| Abstract: | Objective: To investigate the effects of exposure to blast underpressure(BUP) on intracellular free Ca~(2 ) concentrations([Ca~(2 )]i) in outer hair cells(OHCs).Methods:The guinea pigs were killed by decapitation following exposure to the moderate BUP.[Ca~(2 )]i in the OHCs was examined with Fluo-3,a fluorescent probe for [Ca~(2 )]i under laser scanning confocal microscope(LSCM).Results: The intensity of fluorescent [Ca~(2 )]i increased two times as much as that in the control group following 8 hours exposure to BUP.Compared with the control group,it still sustained at a higher level following 24 hours and three days exposure.The above changes were found to be consistent with the increase of auditory brainstem response(ABR) threshold.Conclusion: It is suggested that exposure to moderate experimental BUP can obviously increase the concentration of [Ca~(2 )]i in OHCs of guinea pigs,which may be one of the main causes of hearing loss by exposure to blast underpressure. |
| Keywords: | blast underpressure trauma calcium ion hair cell guinea pig |
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