| 高效液相色谱法测定芍药汤中盐酸小檗碱的含量 |
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| 引用本文： | 陈琳,刘丽梅,王瑞海,牛燕梅.高效液相色谱法测定芍药汤中盐酸小檗碱的含量[J].中国中医药信息杂志,2010,17(3):47-48. |
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| 作者姓名： | 陈琳 刘丽梅 王瑞海 牛燕梅 |
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| 作者单位： | 中国中医科学院中医基础理论研究所,北京,100700 |
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| 基金项目： | 中国中医科学院基本科研业务费自主选题项目 |
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| 摘 要： | 目的建立芍药汤中盐酸小檗碱含量的测定方法。方法采用高效液相色谱法,色谱柱:AgilentXDBC18(4.6mm×250mm,5μm);流动相:乙腈-0.05mol/L磷酸二氢钾水溶液-三乙胺(30∶70∶0.14);流速:1.0mL/min;柱温:25℃;检测波长:345nm。结果盐酸小檗碱在0.07～1.40μg范围呈良好的线性关系,平均回收率为98.63%,RSD=1.21%(n=6),盐酸小檗碱的平均含量为1.55%。结论本法准确、灵敏、重复性好,为芍药汤的质量控制提供了依据。
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| 关 键 词： | 芍药汤 盐酸小檗碱 高效液相色谱法 含量测定 |
Determination of Berberine Hydrochloride in Shaoyao Decoction by HPLC |
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| Institution: | CHEN Lin, LIU Li-mei, WANG Rui-hai, et al (Institute of Basic Theory, China Academy of Chinese Medical Sciences, Beijing 100700, China) |
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| Abstract: | Objective To determine the content of berberine hydrochloride in Shaoyao decoction. Methods HPLC was performed on an Agilent XDB C18 column by using acetonitrile-0.05 mol/L potassium dihydrogen phosphate-triethylamine （30∶70∶0.14） as mobile phase at a flow rate of 1.0 mL/min, the column temperature was 25 ℃ and the UV detection wavelength was 345 nm. Results Berberine hydrochloride showed a good linearity redationship in the range of 0.07～1.40 μg. The average recovery was 98.63% （n=6, RSD=1.21%）. The average content was 1.55%. Conclusion The method is simple and accurate, with good repeatability, and can be used for the quality control of Shaoyao decoction. |
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| Keywords: | Shaoyao decoction berberine hydrochloride HPLC content determination [1] |
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