小鼠HCV皮下移植瘤的建立及DNA 疫苗的治疗作用

目的　建立小鼠丙型肝炎病毒（HCV）皮下移植瘤,并以其为HCV感染动物模型,观察HCV核心(C)基因DNA疫苗（pcDNA-HCV-C）在体内对HCV感染的治疗作用。方法　将pcDNA-HCV-C用脂质体（LipofectAMINE）法转染BALB/c小鼠骨髓瘤Sp2/0（H-2

Inhibition of transplanted tumor producing HCV C protein in mice by DNA vaccine

Objective To establish the transplanted tumor producing HCV Cprotein in mice and study the therapeutic effect of DNA vaccine on HCV infection. Methods The recombinant plasmid pcDNA-HCV-C containing HCV C gene was transfected into mouse myeloma Sp2/0 cells with LipofectAMINE. The transfected Sp2/0 cells were cultured in the selective medium containing G418 (400μg/ml) and the Sp2/0-HCV-C cells expressing HCV C antigen were subcutaneously injected into BALB/c mice. Three days later, the inoculated mice were treated with different agents-pcDNA-HCV-C and pcDNA3 and saline as control. Results The tumorigenicity of Sp2/0-HCV-C cells was significantly decreased after treatment with pcDNA-HCV-C as compared with parental tumor cells and mock vector transfected Sp2/0 cells. Moreover, application of the pcDNA-HCV-C plasmid could prolong the survival time of mice. Conclusion DNA vaccination might function as a candidate biotherapy for the treatment of human HCV infectious.