连接酶链反应与培养法检测沙眼衣原体的比较

目的 比较连接酶链反应（ＬＣＲ）与培养法检测沙眼衣原体（ＣＴ）在泌尿生殖道感染中的临床意义。方法 应用ＬＣＲ对４０２份门诊患者的尿标本进行ＣＴ检测，其中２２６份取尿道拭子（男）或宫颈拭子（女）同时用培养法和ＬＣＲ检测ＣＴ，对两种方法的检测结果进行了比较分析。结果 ＬＣＲ法检测４０２份尿标本，ＣＴ阳性率为２８．３％（１１４／４０２），２２６份拭子标本用ＬＣＲ法检测，阳性率为２７．０％（６１／２２６）

Ligase chain reaction and cell culture in detecting chamydia trachomatis

Objective To study the clinical significance of Ligase chain reaction (LCR) in urogenital infection, we identified chlamydia trachomatis (CT) by LCR and cell culture.Method 402 urine specimens (FVU: first void urine) were identified by LCR, while urinary tract specimens (US) of males and cervical specimens (CS) of females were collected for detecting chlamydia trachomatis by the two methods.Results 402 Urine specimens were identified by LCR, with a positive rate of 28.3% (114/402). In 226 urogenital secretion samples, the positive rate was 27.0%(61/226) and 11.1%(25/226) by using LCR and cell culture respectively ( P <0.005).Conclusion Compared with cell culture LCR is simple, sensitive and suitable for clinical application LCR for detecting chlamydia trachomatis in urine specimens is of clinical significance in diagnosis of urogenital infection.