骨折挫伤胶囊中血竭素的含量测定研究

目的：建立骨折挫伤胶囊中血竭素的含量测定方法。方法：采用高效液相色谱法,色谱柱：C18柱,乙腈-0．05mol／L磷酸二氢钠溶液（45：55）为流动相,流速1．0ml／min,检测波长为440hm,柱温40℃。结果：血竭素在0．01μg～0．06μg范围内呈良好线性关系,平均加样回收率为97．82％,RSD为1．0％（n=6）。结论：本法专属性强,灵敏度高,重现性好,可用于骨折挫伤胶囊的质量控制。

Study on Determination of Dracorhodin in Guzhecuoshang Capsules

Objective：To establish a method for determination of Dracorhodin in Guzhecuoshang Capsules. Methods：The contents of Dracorhodin were determined by HPLC. A Spherixorb C18 column was used with acetoni and 0.05% sodium dihydrgenj phosphate solution （45： 55） as mobile phase. The flow rate was 1.0ml/min and detection wavelength 440nm. The column temperature was 40℃. Results： Dracorhodin showed a good linear relationship at the range of 0.01 -0.061μg. The average recovery rate was 97.82%, and RSD was 1.0% （n = 6）. Conclusion：The method is sensitive, with strong specificity and reproducibility, and can be used for quality control of Guzhecuoshang Capsules.