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Immunochemical studies on mouse myeloma proteins with specificity for dextran or for levan
Authors:A Lundblad  R Steller  E A Kabat  J W Hirst  M G Weigert  M Cohn
Affiliation:1. Department of Microbiology, Neurology and Human Genetics and Development, Columbia University, The Neurological Institute, Presbyterian Hospital, New York, USA;2. Salk Institute for Biological Studies, San Diego, California, U.S.A.;1. Center for Lymphoma & Myeloma, Weill Cornell Medicine/New York-Presbyterian Hospital, New York, NY;2. Division of Hematology/Oncology, Department of Internal Medicine, Meharry Medical College, Nashville, TN;3. Division of Hematology, University Vita-Salute San Raffaele IRCCS San Raffaele Scientific Institute, Milano, Italy;4. Global Medical Affairs, Pfizer Inc, New York, NY;1. BioArctic AB, Warfvinges väg 35, SE-112 51, Stockholm, Sweden;2. Department of Public Health and Caring Sciences/Geriatrics, Uppsala University, Rudbeck Laboratory, Dag Hammarskjölds väg 20, SE-751 85, Uppsala, Sweden;1. Cancer Sciences, Faculty of Medicine, University of Southampton, Somers Cancer Research Building, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK;2. University Surgery, South Academic Block, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK;3. School of Medicine, University of Southampton, University Road, Southampton SO17 1BJ, UK;1. Laboratorio de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Mataojo 2055, 11400 Montevideo, Uruguay;2. Centro de Investigação Translacional em Oncologia, Instituto do Câncer do Estado de São Paulo, Av. Dr. Arnaldo, 251, São Paulo 01246-903, Brazil;3. Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Facultad de Medicina, Universidad de la Republica, Av. Alfredo Navarro 3051, 11600 Montevideo, Uruguay;4. Centro de Medicina Nuclear, Faculdade de Medicina, Universidade de São Paulo, Trav. R. Dr. Ovidio Pires de Campos s/n, São Paulo 05403-010, Brazil;5. Centro de Medicina Nuclear, Hospital de Clínicas, Facultad de Medicina, Universidad de la Republica, Av Italia s/n, 11600 Montevideo, Uruguay;6. Grupo de Química Medicinal, Laboratorio de Química Orgánica, Facultad de Ciencias-Facultad de Química, Universidad de la República, Iguá 4225, 11400 Montevideo, Uruguay;1. Division of Biotechnology Review and Research-III, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA;2. Laboratory of Immunology, National Eye Institute, NIH, Bethesda, MD 20892, USA;3. Laboratory of Emerging Pathogens, Division of Emerging and Transfusion Transmitted Diseases, Office of Blood Research and Review, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD 20993, USA
Abstract:Two mouse myeloma proteins have been studied immunochemically. One has specificity for α(1 → 3) linked dextrans and inhibition studies with α (1 → 3) glucosyloligosaccharides (nigerodextrins) indicate that the size of the combining site is that of a pentasaccharide. The other protein reacts with levan and to a lesser extent with inulin. The best inhibitor was a terminal non-reducing chain of two β (2 → 1) linked fructofuranosyl residues plus an additional β linkage either to carbon 6 of glucose or to carbon 1 of fructose.
Keywords:
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