| 家蝇幼虫、蛹、成虫抗菌肽的聚丙烯酰胺凝胶电泳分析 |
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| 引用本文： | 张庆华,;李飞栋,;赵瑞君,;侯玉英. 家蝇幼虫、蛹、成虫抗菌肽的聚丙烯酰胺凝胶电泳分析[J]. 广东寄生虫学会年报, 2007, 0(3): 207-208 |
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| 作者姓名： | 张庆华, 李飞栋, 赵瑞君, 侯玉英 |
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| 作者单位： | [1]山西医科大学寄生虫学教研室,太原030001; [2]山西省肿瘤医院,太原030013 |
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| 基金项目： | 山西省科技开发基金（晋教计财[2003]46）. |
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| 摘 要： | 目的分析家蝇幼虫、蛹、成虫的抗菌肽成分。方法通过针刺感染大肠杆菌诱导家蝇各虫态产生抗菌肽，再经研磨、离心等步骤提取这些抗菌肽，用改进的聚丙烯酰胺凝胶电泳使其分离。结果家蝇幼虫有7条带，成虫有5条，蛹只有4条。结论家蝇幼虫和成虫的抗菌肽种类较蛹的多，且有一些小分子肽段，可能与其较强的抗菌效果有关；家蝇蛹处于相对静止期，其电泳图中抗菌肽条带较少，无较小的条带，抗菌效果可能较幼虫和成虫的差。
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| 关 键 词： | 家蝇 抗菌肽 电泳 |
Polyacrylamide Gel Electrophoretic Analysis of Antimicrobial Peptides in Musca domestica |
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| Affiliation: | ZHANG Qing-hua, LI Fei-dong, ZHAO Rui-jun, HOU Yu-ying(1.Department of Parasitology, Shanxi Medical University, Taiyuan 030001 ;2.Tumor Hospital of Shanxi Province, Taiyuan 030013, China) |
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| Abstract: | Objective To analyze the antimicrobial peptides from larvae,pupae and adults of Musca domestica Methods Antimicrobial peptides were induced by infection the insects with bacteria. The peptideS were extracted, and analyzed using an improved tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis （Tricine-SDS-PAGE） method. Results A total of 7, 4, and 5 different antimicrobial peptides were identified in the larvae, pupae, and the adults, respectively. Conclusion The presence of a large number of antimicrobial peptides in the larvae and adults is correlated with the resistance of this organism. |
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| Keywords: | Musca domestica antimicrotial peptides electrophoresis |
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