| 干扰素基因定向进化库的建立及初步筛选 |
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| 引用本文： | 吴明福,;蒋忠军,;马玉海,;唐博恒,;王君伟.干扰素基因定向进化库的建立及初步筛选[J].广东寄生虫学会年报,2007(5):426-428. |
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| 作者姓名： | 吴明福 ;蒋忠军 ;马玉海 ;唐博恒 ;王君伟 |
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| 作者单位： | [1]东北农业大学,哈尔滨150030; [2]广州军区疾病预防控制中心,广州510069 |
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| 基金项目： | 广州市科技攻关项目（No.200223-B4022）. |
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| 摘 要： | 目的构建α干扰素基因家族定向文库。方法利用D NAshuffling技术对人、鸡和猪α干扰素基因进行人工改造，结合噬菌体展示技术构建噬菌体基因改组文库，并对文库进行验证。结果构建了α干扰素基因家族定向文库，其容量为3．5×10^7pfu；测序得到了3条与猪、人α干扰素基因高度同源的序列。结论得到了1个高质量的α干扰素基因家族定向文库，为今后筛选高效的α干扰素奠定了基础。
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| 关 键 词： | α干扰素 DNA改组 文库 |
Established and Screened of Directed Evolution Library of Interferon Gene in Swine |
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| Institution: | WU Ming-fu, JIANG Zhong-jun, MA Yu-hai, TANG Bo-heng, WANG Jun-wei (1. Northeast Agricultural University, Harbin 150030; 2.The Center for Disease Control and Prevention of PLA, Guangzhou 510069, China) |
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| Abstract: | Objective To construct a library of α-interferon gene. Methods A library of human, swine, and avian interferon gene was constructed using both DNA shuffling technique and phage display technology. Results The titers of un-amplified and the amplified library are 3.5 ×10^7 pfu/ml,respectively. 3 of the human interferon DNA sequences show a high degree of homology with pig interferon. Conclusion A high quality interferon gene library was constructed successfully. |
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| Keywords: | α-interferin DNA shuffling library |
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