| FQ—PCR测定HBV DNA与ELISA检测HBV—M相关性分析 |
| |
| 引用本文： | 雷秀霞,;李国豪,;明凯华,;陈小辉. FQ—PCR测定HBV DNA与ELISA检测HBV—M相关性分析[J]. 广东寄生虫学会年报, 2007, 0(3): 239-240 |
| |
| 作者姓名： | 雷秀霞, 李国豪, 明凯华, 陈小辉 |
| |
| 作者单位： | [1]广州市第一人民医院,广州510180 |
| |
| 摘 要： | 目的探讨HBV DNA含量与HBsAgS／CO值之间的相关性。方法分别用荧光定量聚合酶链反应（FQ-PCR）法和酶联免疫吸附试验（ELISA）检测100例乙肝患者的HBV DNA含量和乙肝两对半．并记录HBsAg S／CO值。结果100例乙肝患者HBV DNA含量对数值为（4．28±1．89），HBsAg S／CO值为（15．09±6．64），两指标间作相关性分析，r=-0．0156，P〉0．05，无相关性。结论HBV DNA是判断乙肝病毒复制的较准确、较直接的抗乙肝病毒疗效观察指标，而HBsAg S／CO值的高低与疾病的严重程度及疗效无关。
|
| 关 键 词： | 乙型肝炎病毒DNA 荧光定量聚合酶链反应 酶联免疫吸附试验 |
A Comparative Study of FQ-PCR and ELISA for the Detection of HBV DNA and HBV-M |
| |
| Affiliation: | LEI Xiu-xia, LI Guo-hao, MING Kai-hua, CHEN Xiao-hui ( The First Municipal People＇s Hospital of Guangzhou, Guangzhou 510180, China) |
| |
| Abstract: | Objective To determine the correlation between the level of HBV DNA and the value of HBsAg S/ CO. Methods 100 serum samples from hepatitis B patients were analyzed for HBV DNA by FQ-PCR and HBsAg by ELISA. The HBsAg S/CO values were also recorded. Results The logarithmic concentration of HBV DNA in these samples was （4.28±1.89） and the HBsAg S/CO value was （15.09±6.64）. There was no correlation between the two values （r=-0.0156, P 〉 0.05 ）. Conclusion HBV DNA is a reliable maker for diagnosis of HBV infection in patients undergoing treatment. There was a poor correlation between the value of HBsAg S/CO and the disease severity. |
| |
| Keywords: | HBV DNA FQ-PCR ELISA |
| 本文献已被 维普 等数据库收录！ |
|
