Simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae by high performance liquid chromatography-evaporative light scattering detection |
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Authors: | Zhang Mingchun Zhang Yanqing Xie Junbo |
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Affiliation: | aDepartment of Pharmaceutical Engineering, Tianjin University of Commerce, Tianjin 300134, China;bTianjin Key Laboratory of Food Biotechnology, Tianjin 300134, China |
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Abstract: | A reverse phase high performance liquid chromatographic (HPLC) method with evaporative light scattering detection (ELSD) was developed for simultaneous determination of jujuboside A, B and betulinic acid in semen Ziziphi spinosae. The analysis was performed by gradient elution, using an aqueous mobile phase (containing 0.1% acetic acid) modified by acetonitrile. The evaporator tube temperature of ELSD was set at 45 °C, and with the nebulizing gas flow-rate of 1.8 l/min. The method was validated for accuracy, reproducibility, precision and limits of detection and quantification. Quantification of the three active compounds in semen Ziziphi spinosae from different locations was performed by this method, which provides a new tool for quality assessment of semen Ziziphi spinosae. |
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Keywords: | Semen Ziziphi spinosae Simultaneous determination Jujuboside A Jujuboside B Betulinic acid HPLC-ELSD |
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