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| 铁皮石斛蔗糖合成酶基因的克隆及表达分析 | |
| 引用本文： | 孟衡玲,段承俐,萧凤回,杨生超,查应红,文国松.铁皮石斛蔗糖合成酶基因的克隆及表达分析[J].中国中药杂志,2011,36(7):833-837. |
| 作者姓名： | 孟衡玲 段承俐 萧凤回 杨生超 查应红 文国松 |
| 作者单位： | 1. 云南农业大学,中药材研究所/云南省中药材规范化种植技术指导中心,云南,昆明,650201 2. 云南农业大学,中药材研究所/云南省中药材规范化种植技术指导中心,云南,昆明,650201;浙江农林大学,林业与生物技术学院,浙江,临安,311300 3. 云南红河群鑫石斛种植有限公司,云南,屏边,661200 |
| 基金项目： | 云南省重大产业技术开发项目 |
| 摘 要： | 目的:克隆铁皮石斛Dendrobium officinale蔗糖合成酶基因并对其表达分析,为研究铁皮石斛多糖合成与蔗糖合成酶活性关系及调控表达提供理论依据.方法:基于GenBank上已知的蔗搪合成酶基因SS同源序列,应用RT-PCR和RACE等方法,克隆铁皮石斛蔗糖合成酶基因全长,将目的片段转化大肠杆菌表达菌株BL21中,IPTG诱导表达,SDS-PAGE电泳分析.结果:成功克隆了铁皮石斛蔗糖合成酶基因,该基因cDNA全长2 424 bp,编码807个氨基酸(命名为DOSS1),登录号HQ856835,该基因氨基酸序列与兰科植物Moluara yellow(AF530568)同源性达到95%,与Oncidium goldiana(AF530567)同源性达到90%,与禾本科植物的SS基因同源性在80%以上.并对其进行大肠杆菌原核表达诱导,诱导的工程菌能明显表达出一条相对分子质量约为92×10(3)左右的蛋白质,与理论相对分子质量基本相符.结论:成功克隆了铁皮石斛蔗糖合成酶基因,并诱导出一条与理论蛋白相对分子质量相符的蛋白条带. |
| 关 键 词： | 铁皮石斛 蔗搪合成酶 基因克隆 表达分析 |
| 收稿时间： | 2010/11/16 0:00:00 |
Molecular cloning and expression analysis of sucrose synthase gene from Dendrobium officinale |
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| MENG Hengling,DUAN Chengli,XIAO Fenghui,YANG Shengchao,Zha Yinghong and WEN Guosong.Molecular cloning and expression analysis of sucrose synthase gene from Dendrobium officinale[J].China Journal of Chinese Materia Medica,2011,36(7):833-837. | |
| Authors: | MENG Hengling DUAN Chengli XIAO Fenghui YANG Shengchao Zha Yinghong and WEN Guosong |
| Institution: | Institue of Chinese Medical Materials, Yunnan Agricultural University/Yunnan Provincial Good Agricultural Practice Center of Chinese Medical Materials, Kunming 650201, China;Institue of Chinese Medical Materials, Yunnan Agricultural University/Yunnan Provincial Good Agricultural Practice Center of Chinese Medical Materials, Kunming 650201, China;Faculty of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an 311300, China;Institue of Chinese Medical Materials, Yunnan Agricultural University/Yunnan Provincial Good Agricultural Practice Center of Chinese Medical Materials, Kunming 650201, China;Faculty of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an 311300, China;Institue of Chinese Medical Materials, Yunnan Agricultural University/Yunnan Provincial Good Agricultural Practice Center of Chinese Medical Materials, Kunming 650201, China;Yunnan honghe Dendrobium planting Co., Ltd., Pianbian 661200, China;Institue of Chinese Medical Materials, Yunnan Agricultural University/Yunnan Provincial Good Agricultural Practice Center of Chinese Medical Materials, Kunming 650201, China |
| Abstract: | |
| Keywords: | Dendrobium officinale sucrose synthase gene cloning expression analysis |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |