| 大胚龄人神经干细胞长期冻存后的复苏培养 |
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| 引用本文： | 邓志锋,涂伟,汪泱,赖贤良,谢安.大胚龄人神经干细胞长期冻存后的复苏培养[J].江西医学检验,2008,26(2):109-110. |
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| 作者姓名： | 邓志锋 涂伟 汪泱 赖贤良 谢安 |
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| 作者单位： | 邓志锋（330006,南昌,南昌大学第二附属医院神经外科）;涂伟（330006,南昌,南昌大学第二附属医院神经外科）;汪泱（330006,南昌,南昌大学第一附属医院泌尿外科研究所）;赖贤良（330006,南昌,南昌大学第二附属医院神经外科）;谢安（330006,南昌,南昌大学第一附属医院泌尿外科研究所） |
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| 基金项目： | 国家自然科学基金资助项目(30560156)江西省自然科学基金资助项目(0540087) |
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| 摘 要： | 目的 探讨长期冻存后的大胚龄人胚神经干细胞(neural stem cells,NSCs)的生长及其生物学特性.方法 分别于冻存后6、12及24个月,快速解冻复苏胚龄为16～20周的胎脑细胞,采用无血清培养液悬浮培养.观察培养细胞的活性及成球时间,采用免疫细胞化学方法鉴定这些细胞球及其分化成熟后的细胞表面标志.结果 培养传代的细胞透亮、成球时问短,与取自新鲜胎脑的神经干细胞无明显区别.细胞球Nestin染色阳性,成熟分化后神经元特异性烯～M(NSE)、胶质纤维酸性蛋白(GFAP.)及2,3',-环腺苷酸-3,-磷酸二酯酶(CNPase)抗原呈阳性.结论 长期冻存后的人大胚龄神经干细胞仍具有较强增殖能力及多分化潜能.
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| 关 键 词： | 神经干细胞 胚胎 冻存 |
| 文章编号： | 1674-1129(2008)02-0109-03 |
Culture of thawing old human embryonic neural stem cells after long cryopreservation |
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| DENG Zhifeng,TU Wei,WANG Yang,et al..Culture of thawing old human embryonic neural stem cells after long cryopreservation[J].Jiangxi Journal of Medical Laboratory Sciences,2008,26(2):109-110. |
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| Authors: | DENG Zhifeng TU Wei WANG Yang |
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| Institution: | DENG Zhifeng,TU Wei,WANG Yang,et al. Department of Neurosurgery,Second Affiliated Hospital of Nanchang University,Nanchang 330006,China |
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| Abstract: | Objective To study the growth and property of thawing old human embryonic neural stem cells(NSCS) after long cryopreservation. Methods After 6,12 and 24 months, the cells isolated from about 16-20 months fetus were thawed and cultured in serum-free medium. The cells were observed and identified with inverted microscopy and immunocytochemistry. Results The cells grew very well after thawing, and presented an ability of consecutive cloning and differentiation. The clusters of cells were positive by Nestin imm... |
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| Keywords: | Neural stem cells Embryo Cryopreservation |
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