低剂量中子照射对大鼠细胞外基质成分的影响

目的 探讨低剂量中子辐射对大鼠肺组织细胞外基质的影响。方法 120只雄性Wistar大鼠随机分为四个组,分别接受0、0.29、0.62和1.20 Gy的²⁵²Cf中子源照射,分别在照后3 d、1个月和2个月时处死动物,取肺组织样品-30℃保存。采用放射免疫方法测定其透明质酸、层粘连蛋白、四型胶原和三型前胶原的含量。结果 照射后各组肺组织透明质酸水平均无明显变化;层粘连蛋白水平在照后3 d,与对照组相比低中高3个剂量组均出现显著降低,直到照后2个月仍未完全恢复到对照组水平;四型胶原水平在照射后均出现升高趋势,但差异无显著性;三型前胶原在照后早期出现升高趋势,但随后降低。结论 中子低剂量照射可引起肺组织某些细胞外基质成分的改变,但效应的形式和程度均随照射剂量及照后时间而不同。

The Effect of Low-dose Neutron Irradiation on Extracellular Matrix

Projective To study the effect of neutron irradiation on extracellular matrix.Methods 120 male wistar rats were divided into four groups at random, and then exposed to neutron of ²⁵²Cf-source at the doses of 0, 0.29, 0.62 and 1.20 Gy, respectively.After the exposure of 3 days, 1 month and 2 months, the rats were sacrificed and lung tissue specimens stored at -30℃.Hyaluronan, laminin, type Ⅲ procollagen and type Ⅳ collagen in the lung tissue were detected by the method of radioimmunoassay.Results The differences of the levels of hyaluronan in lung tissue among the groups were unsignificant.The levels of laminin in 0.29, 0.62 and 1.20 Gy groups after the 3-day exposure were remarkably different to those of the control group, and unable to recover completely even 2 months after the exposure.The levels of type Ⅳ collagen higher three irradiated groups were all higher, but not significantly.The levels of type Ⅲ procollagen in the early stage after exposure higher, and later they lowered.Conclusion The levels of some components of extracellular matrix in the lung tissue of rat can be changed by low-dose of neutron irradiation, but their variational modes and degrees depend on the dose of neutron irradiation and the length of period after exposure.