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| 高效液相色谱法测定水杨酸醇溶液中水杨酸含量 | |
| 引用本文： | 周蓓瀛,李楠,张钢平,李莉,郄冰冰.高效液相色谱法测定水杨酸醇溶液中水杨酸含量[J].中国药业,2009,18(16):38-39. |
| 作者姓名： | 周蓓瀛 李楠 张钢平 李莉 郄冰冰 |
| 作者单位： | 1. 冀中能源峰峰集团总医院药剂科,河北,邯郸,056200 2. 河北省邯郸市铁路医院,河北,邯郸,056000 3. 河北省邯郸市药品检验所,河北,邯郸,056001 4. 河北省药品检验所,河北,石家庄,050011 |
| 摘 要： | 目的采用高效液相色谱（HPLC）法测定水杨酸醇溶液中水杨酸的含量。方法以Eclipse XDB—C18柱（250mm×4．6mm，5μm）为色谱柱，以甲醇-0．4％磷酸溶液（58：42）为流动相，检测波长为298nm，流速为1．0mL／min，柱温为25℃。结果水杨酸进样量在0．16-0．80μg范围内与峰面积积分值呈良好的线性关系，r=1．0000（12=6），平均回收率为99．51％，RSD为0．2％（n=6）。结论HPLC法简单、结果准确、灵敏度高，可用于水杨酸醇溶液中水杨酸的含量测定。 |
| 关 键 词： | 水杨酸醇溶液 水杨酸 高效液相色谱法 |
Determination of Content of Salicylic Acid in Salicylic Acid Alcoholic Solution by HPLC |
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| Zhou Beiying,Li Nan,Zhang Gangping,Li li,Qie Bingbing.Determination of Content of Salicylic Acid in Salicylic Acid Alcoholic Solution by HPLC[J].China Pharmaceuticals,2009,18(16):38-39. | |
| Authors: | Zhou Beiying Li Nan Zhang Gangping Li li Qie Bingbing |
| Institution: | 1. Department of Pharmacy, General Hospital, Jizhong Energy Fengfeng Group, Handan, Hebei, China 056200; 2. Handan Train Systemic Hospital, Handan, Hebei, China 056000; 3. Handan Institute for Drug Control, Handan, Hebei, China 056001; 4. Hebei Provincial Institute for Drug Control, Shifiazhang, Hebei, China 050011) |
| Abstract: | Objective To develop a HPLC method for determining the content of salicylic acid in Salicylic Acid Alcoholic Solution. Methods The determination was performed on the Eclipse XDB C18 column, using methylalcohol-0. 4% phosphoric acid solution（58：42）as the mobile phase at the flow rate of 1.0 mL/min and the detection wavelength at 298 nm. The column temperature was 25 ℃. Results The linear relation of the peak area of salicylic acid was determined as 0.16-0.80 μg by HPLC with the regression coefficient of 1. 000 0 and the average recovery rate of 99.51%, RSD = 0. 2% （n = 6）. Conclusion The method is simple, rapid and accurate. It can be used for determining salicylic acid in Salicylic Acid Alcoholic Solution. |
| Keywords: | salicylic acid Salicylic Acid Alcoholic Solution HPLC |
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